Pelagicola litoralis gen. nov., sp. nov., isolated from coastal water in Korea

Abstract

A Gram-negative, strictly aerobic, non-motile, club-shaped bacterial strain, designated CL-ES2(T), was isolated from coastal water from the east coast of Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain CL-ES2(T) was related to the genera Phaeobacter (95.0-96.6% similarity to the type strains), Leisingera (96.1%) and Marinovum (95.6%) in the family Rhodobacteraceae. However, strain CL-ES2(T) did not form a robust clade with any species of the Roseobacter clade, instead forming a distinct subline. The optimum temperature and pH for growth were 25 degrees C and pH 7. Strain CL-ES2(T) was able to grow with sea salts at concentrations in the range 2-6%, with optimum growth occurring at 3-4%. The major fatty acid was C(18:1)omega7c (75.2%). The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and three unidentified lipids. The isoprenoid quinone was Q-10. The G+C content of the DNA was 47.0 mol%. On the basis of the data from the polyphasic analysis, strain CL-ES2(T) represents a novel genus and species, for which the name Pelagicola litoralis gen. nov., sp. nov. is proposed. The type strain of Pelagicola litoralis is CL-ES2(T) (=KCCM 42274(T) =DSM 18290(T)).