Pelagibaca abyssi sp. nov., of the family Rhodobacteraceae, isolated from deep-sea water.

Abstract

A Gram-stain negative, oval-shaped, aerobic, catalase and oxidase-positive bacterium, designated JLT2014(T), was isolated from a deep-seawater sample (obtained at a 2,000 m depth) of the Southeastern Pacific Ocean. The dominant fatty acids were identified as C18:1ω7c/C18:1ω6c, C16:0 and C10:0 3-OH, which altogether represented 60.1 % of the total. The predominant respiratory quinone was identified as Q-10. The G+C content of genomic DNA was determined to be 66.4 mol %. The major polar lipids were identified as phosphatidylethanolamine and diphosphatidylglycerol. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate can be affiliated with the Roseobacter clade within the family Rhodobacteraceae. Strain JLT2014(T) exhibited highest 16S rRNA gene sequence similarity value to Pelagibaca bermudensis HTCC2601(T) (sequence similarity value: 97.6 %). The DNA-DNA relatedness value between strain JLT2014(T) and P. bermudensis HTCC2601(T) was 46.9 ± 2 %. Based on phenotypic properties and phylogenetic analysis, the name Pelagibaca abyssi sp. nov. is proposed, with JLT2014(T)(=LMG 27363(T)=CGMCC 1.12376(T)) as the type strain.