Abstract
PEG-papain catalyzed synthesis of a Kyotorphin derivative has been investigated in aqueous organic solvents using Bz-Tyr-OEt and Arg-OMe as substrates. PEG-papain has shown increased resistance to high pH and high ethanol concentration; 42 % of Bz-Try-OEt has been converted to Bz-Tyr-Arg-OH under the optimized reaction condition (pH 7.0–9.0; 40 % EtOH).
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