不同温度下PEG-6000模拟干旱对柠条锦鸡儿种子萌发的胁迫效应

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 不同温度下PEG-6000模拟干旱对柠条锦鸡儿种子萌发的胁迫效应 DOI: 10.5846/stxb201409021746 作者: 作者单位: 北方民族大学生物科学与工程学院,北方民族大学生物科学与工程学院,北方民族大学生物科学与工程学院,北方民族大学生物科学与工程学院,宁夏白芨滩国家级自然保护区管理局,宁夏白芨滩国家级自然保护区管理局 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金资助项目(31260151) Stress effects of simulated drought by polyethylene glycol on the germination of Caragana korshinskii Kom. seeds under different temperature conditions Author: Affiliation: College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan Ningxia,College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan Ningxia,College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan Ningxia,College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan Ningxia,Administration Bureau of Baijitan National Nature Reserve of Ningxia,Administration Bureau of Baijitan National Nature Reserve of Ningxia Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:在人工气候箱控制的不同温度(10、15、20、25℃和30℃)下,设置聚乙二醇(PEG-6000)质量百分比浓度分别为0%、5%、10%、15%、20%、25%和30%的7个模拟干旱胁迫处理,研究了柠条锦鸡儿(Caragana korshinskii Kom.)种子的萌发特性。结果表明:(1) 温度和PEG模拟干旱胁迫及二者的交互作用对柠条锦鸡儿种子的萌发率、萌发速率系数、萌发值、萌发指数和活力指数均具有极显著影响(P < 0.01)。(2) 种子萌发率在15℃下最大,显著大于10℃下的(P < 0.01),随着温度的继续升高逐渐减小;萌发速率系数随着温度的升高有减小的趋势,30℃下又显著增大;萌发值和萌发指数分别在25℃和20℃下最大,但均随温度的继续升高而显著减小(P < 0.05);活力指数随着温度的升高而减小,除在10℃和20℃间无显著差异外,其他温度间均差异显著(P < 0.05)。(3) 种子萌发率、萌发值和萌发指数在15℃下与PEG浓度呈不显著的负相关关系,而在其他温度下均与PEG胁迫浓度显著负相关(P < 0.05),且相关系数均在25℃或30℃下最大;萌发速率系数在各温度下均与PEG胁迫浓度显著正相关;活力指数与PEG胁迫浓度呈显著负相关,相关系数随着温度的升高逐渐增大。(4) 萌发参数与PEG胁迫浓度间的回归模型表现为三次曲线、二次曲线和直线模型3种类型,其中以三次曲线模型为主,全部回归模型均达到显著性水平(P < 0.05);在20℃以下的温度条件下,较低浓度PEG处理对柠条锦鸡儿种子萌发具有一定的促进作用,在15℃下种子萌发对PEG模拟干旱胁迫的耐受性最强;柠条锦鸡儿种子在不同温度和干旱胁迫下的萌发模式是对其分布区生境长期适应和进化的结果。 Abstract:In this study, we investigated the effect of drought on the germination characters of Caragana korshinskii Kom seeds under different temperature conditions. We carried out an experiment with 5 different constant temperatures (10℃, 15℃, 20℃, 25℃, and 30℃) controlled by artificial climate incubators and 7 simulated drought stress conditions created by different polyethylene glycol (PEG)-6000 concentrations (0%, 5%, 10%, 15%, 20%, 25%, and 30%). The results revealed that temperature, simulated drought stress by PEG, and their interaction had a significant impact on the germination percentage (GP), coefficient of germination rate (CGR), germination value (GV), germination index (GI), and vigor index (VI) of C. korshinskii Kom. seeds (P < 0.01). Seeds at 15℃ showed the maximum GP that was significantly higher than that of seeds at 10℃ (P < 0.01) and declined gradually as the temperature increased. CGR decreased (i.e., acceleration of germination schedule) with increasing temperature and a significant increase was identified at 30℃. The highest GV and GI were detected at 25℃ and 20℃, respectively, but both of them declined significantly with continued temperature increasing (P < 0.05). VI decreased with increasing temperature and a significant difference was detected among all treatments at different temperatures (P < 0.05), except for that between 10℃ and 20℃. The relationships between GP, GV, GI, and PEG concentrations at 15℃ were insignificantly negative, while the negative correlations were significant at all other temperatures (P < 0.05) and the correlation coefficients maximized at 25℃ or 30℃. It was found that CGRs were positively correlated with the concentration of PEG at all temperatures. A significant negative correlation between VI and PEG concentration was identified and the correlation coefficient increased with increasing temperature. The regression models of germination parameters and PGE concentrations presented cubic curves, quadratic curves, and linear model and all models were significant (P < 0.05). Below 20℃, lower concentration of PEG partly facilitated the germination of C. korshinskii Kom. seeds. The highest tolerance of seed germination to simulated drought stress of PEG was detected at 15℃. The germination pattern of C. korshinskii Kom. seeds to temperature and drought stress could be the result of long-term adaptation and evolution to the conditions of its natural distributing region. 参考文献 相似文献 引证文献