Pedobacter ruber sp. nov., a psychrophilic bacterium isolated from soil

Abstract

A Gram-staining-negative, red-pigmented, psychrophilic, non-motile and rod-shaped bacterial strain, designated W1(T), was isolated from soil and subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W1(T) grouped with members of the genus Pedobacter and formed a distinct cluster with the type strain of Pedobacter composti (97.8 % 16S rRNA gene sequence similarity). Levels of 16S rRNA gene sequence similarity between strain W1(T) and the type strains of all other recognized species of the genus Pedobacter available at the time of writing were <97.0 %. The predominant cellular fatty acids (≥ 10 %) of strain W1(T) were summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c) and anteiso-C(15 : 0). The only menaquinone was MK-7. The genomic DNA G+C content was 39.0 mol% (HPLC). Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain W1(T) is a representative of a novel species of the genus Pedobacter, for which we propose the name Pedobacter ruber sp. nov. The type strain is W1(T) ( = DSM 24536(T) = LMG 26240(T)).