Pedobacter boryungensis sp. nov., isolated from soil

Abstract

A Gram-negative, non-sporulating, non-flagellated rod, designated BR-9(T), was isolated from soil collected on the Korean peninsula. Strain BR-9(T) grew optimally at pH 6.0-7.0, at 30 °C and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BR-9(T) belonged to the genus Pedobacter and clustered with Pedobacter insulae DS-139(T) and Pedobacter koreensis WPCB189(T). Strain BR-9(T) exhibited 98.2 and 97.5% 16S rRNA gene sequence similarity with P. insulae DS-139(T) and P. koreensis WPCB189(T), respectively, and <96.7% sequence similarity with the type strains of other species in the genus Pedobacter. Strain BR-9(T) contained MK-7 as the predominant menaquinone and iso-C(15:0) and summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) as the major fatty acids. The DNA G+C content of strain BR-9(T) was 38.5 mol%. DNA-DNA relatedness between strain BR-9(T) and P. insulae DS-139(T) and P. koreensis KCTC 12536(T) was 3.4-4.2%, which indicated that the isolate was genetically distinct from these type strains. Strain BR-9(T) was also distinguishable by differences in phenotypic properties. On the basis of the data presented, strain BR-9(T) is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter boryungensis sp. nov. is proposed. The type strain is BR-9(T) (=KCTC 23344(T) =CCUG 60024(T)).