Pediococcus pentosaceus IM96 Exerts Protective Effects against Enterohemorrhagic Escherichia coli O157:H7 Infection In Vivo.

Qinghua Ye,Juan Wang,Shuhong Zhang,Xinqiang Xie,Haixin Li,Hui Zhao,Jumei Zhang,Shi Wu,Runshi Yang,Lei Wu,Yu Ding,Ying Li,Moutong Chen,Qingping Wu,Tingting Liang,Liang Xue

doi:10.3390/foods10122945

Abstract

Enterohemorrhagic Escherichia coli (EHEC) is a notorious and prevalent foodborne pathogen which can cause serious intestinal diseases. The antagonistic activity of probiotics against EHEC is promising, but most of the studies concerning this subject have been carried out in vitro. Specifically, the interaction between Pediococcus pentosaceus and EHEC O157:H7 in vivo has not been reported yet. In this study, we investigated the protective effect of P. pentosaceus IM96 on EHEC O157:H7-infected female mice in vivo. The results demonstrated that P. pentosaceus IM96 reduced the level of pro-inflammatory factors and increased the level of anti-inflammatory factors of EHEC O157:H7-infected mice. Furthermore, P. pentosaceus IM96 alleviated intestinal mucosal damage and increased the level of MUC-2, tight junction (TJ) proteins, and short chain fatty acids (SCFAs). The intestinal microbial community structure and the diversity and richness of the microbiota were also changed by P. pentosaceus IM96 treatment. In summary, P. pentosaceus IM96 exerted protective effects against EHEC O157:H7 via alleviating intestinal inflammation, strengthening the intestinal barrier function, and regulating intestinal microbiota, suggesting that P. pentosaceus IM96 might serve as a potential microbial agent to prevent and treat intestinal diseases caused by EHEC O157:H7 infection in the future.

Highlights

To verify the antibacterial activity of P. pentosaceus IM96, we examined the activity by using the oxford cup agar diffusion method
Consistent with these findings, we found that Escherichia coli (EHEC) O157: H7 infection could greatly decrease the concentration of MUC-2 and the number of goblet cells in the jejunum, but the intake of P. pentosaceus
P. pentosaceus IM96 is capable of selectively elevating the abundance of beneficial bacteria and decreasing the prevalence of pathogens, these results suggested the beneficial effect of P. pentosaceus IM96 functions through regulating the intestinal microbiota

Summary

Introduction

Numerous studies have shown that probiotics have an antagonistic effect on pathogenic bacteria. Bioengineered Lacticaseibacillus casei prevented foodborne Listeria monocytogenes infection through competitive exclusion, maintenance of intestinal epithelial barrier functions, and contact-dependent immunomodulation [7]. Probiotics and their metabolites, as potential antibacterial agents, have attracted great interest in the treatment of diseases caused by pathogenic bacteria. P. pentosaceus is a facultative anaerobic lactic acid bacterium that can affect carbohydrate degradation It is a promising strain with valuable potentials [8]. This study explored the protective effect of P. pentosaceus IM96 on EHEC O157:H7-infected female mice in vivo

Materials and Methods

Antibacterial Ability Evaluation

Animals and Grouping

Intestinal Morphology Observation

Immunohistochemistry

Quantification of SCFAs

Intestinal Microbiota Analysis

2.10. Statistical Analysis

Results

Discussion

Conclusions