Pediococcus acidilactici CECT9879 (pA1c) Counteracts the Effect of a High-Glucose Exposure in C. elegans by Affecting the Insulin Signaling Pathway (IIS).

Deyan Yavorov-Dayliev,María Oneca,Fermín I Milagro,Paula Aranaz,Josune Ayo

doi:10.3390/ijms23052689

Abstract

The increasing prevalence of metabolic syndrome-related diseases, including type-2 diabetes and obesity, makes it urgent to develop new alternative therapies, such as probiotics. In this study, we have used Caenorhabditis elegans under a high-glucose condition as a model to examine the potential probiotic activities of Pediococcus acidilactici CECT9879 (pA1c). The supplementation with pA1c reduced C. elegans fat accumulation in a nematode growth medium (NGM) and in a high-glucose (10 mM) NGM medium. Moreover, treatment with pA1c counteracted the effect of the high glucose by reducing reactive oxygen species by 20%, retarding the aging process and extending the nematode median survival (>2 days in comparison with untreated control worms). Gene expression analyses demonstrated that the probiotic metabolic syndrome-alleviating activities were mediated by modulation of the insulin/IGF-1 signaling pathway (IIS) through the reversion of the glucose-nuclear-localization of daf-16 and the overexpression of ins-6 and daf-16 mediators, increased expression of fatty acid (FA) peroxisomal β-oxidation genes, and downregulation of FA biosynthesis key genes. Taken together, our data suggest that pA1c could be considered a potential probiotic strain for the prevention of the metabolic syndrome-related disturbances and highlight the use of C. elegans as an appropriate in vivo model for the study of the mechanisms underlying these diseases.

Highlights

Obesity is defined as a pathological state characterized by an excessive accumulation or abnormal distribution of body fat [1,2]
It was observed that pA1c reduced fat accumulation in wild-type C. elegans in a nematode growth medium (NGM) medium (8.8%) and in a glucose-loaded (10 mM)
In a glucose-loaded (10 mM) NGM medium compared with control worms (Figure 1D), quantified by Oil Red O (ORO)

Summary

Introduction

Obesity is defined as a pathological state characterized by an excessive accumulation or abnormal distribution of body fat [1,2]. It is considered a chronic disease with a multifactorial origin that produce oxidative stress and a proinflammatory condition in the patient. There is no specific treatment against obesity and not all people respond in the same way to the treatment For that reason, it has become urgent and necessary for the development of novel and alternative therapies to reduce the progression of this pathology, and at the same time, prevent metabolic syndrome-related diseases like type-2 diabetes

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Results

Conclusion