Pedigree and Molecular Analyses in the Assessment of Genetic Variability of the Polish Greyhound

Abstract

Simple SummaryThe Polish Greyhound is an old Polish breed. The first descriptions of hunting with greyhounds can be found in the hunting literature of the 16th century, and the first detailed description of a greyhound comes from 1600. The Polish Greyhound probably originates from the Saluki-type Asian greyhounds. The population of this breed is very small and limited mainly to the territory of its country of origin (Poland). Pedigree books were opened for this breed in 1981. The narrow gene pool necessitated mating relatives, resulting in the appearance of various genetic diseases in the breed. The analysis of Polish Greyhound pedigrees registered in the pedigree books from the time of the registration of the breed and molecular tests facilitated calculation of the degree of relatedness in the breed and to design future breeding plans, taking into account that a 2.56% increase in inbreeding per complete generation will be observed if the current breeding program and similar matings are maintained.The aim of the study was to assess the genetic variability of the Polish Greyhound population based on pedigree analysis and molecular DNA testing and to determine the degree of relatedness among individuals in the population. Pedigree data of 912 Polish Greyhounds recorded in pedigree books since they were opened for this breed were analyzed. For molecular testing, DNA was obtained from cheek swabs taken from 235 dogs of the tested breed. A panel of 21 markers (Short Tandem Repeat—STR) was used. The mean inbreeding determined for the Polish Greyhound population based on pedigree analyses was low and amounted to 11.8%, but as many as 872 individuals of the 912 dogs in the studied population were inbred. A total of 83 founders (at least one unknown parent) were identified, among which 27 founders had both unknown parents. Full-sibling groups consisted of 130 individuals, with a minimum and maximum litter size of 2 and 16, respectively. The average litter size was 5.969. Gene diversity calculated based on the mean kinship matrix was 0.862 and the population mean kinship was 0.138. The founder genome equivalent based on the mean kinship matrix was 3.61; the founder genome surviving level was 12.34; the mean Ne was estimated at 21.76; and the Ne/N ratio was 0.135. The FIS inbreeding coefficient for 21 STR was negative, and the mean FIS value for all loci had a low negative value (−0.018). These values suggest a low level of inbreeding in the examined breed as well as the avoidance of mating related animals.