Abstract
Purpose : to estimate the expression of a molecule of vascular adhesion of PECAM-1 (CD31) at patients with acute pancreatitis of varying severity in ΙА phase diseasedevelopment. Materials and methods . Work has clinical character and is based on the prospective analysis of observation and treatment of 135 patients with acute pancreatitis of various degrees of severity. Patients were divided into three groups, depending on severity of a disease: 1 group — 45 patients with slight acute pancreatitis, the 2 nd group — 45 patients with medium-weight acute pancreatitis, the 3 rd group — 45 patients with heavy acute pancreatitis. Blood sampling for a research was carried out at receipt prior to therapy. Phenotyping of lymphocytes was carried out by method of an indirect immunofl uorescence by means of mouse monoclonal antibodies to the molecules CD31 receptors of lymphocytes. Results . The obtained data demonstrate that the percent of CD31-expressing of lymphocytes depends on weight of acute pancreatitis. At initially severe form of a disease, there was strengthening of process of transendothelial migration of lymphocytes in the center of forming of a necrosis in pancreas tissue. In our research signifi cantly (p <0.001, p1 <0.001, p2 <0.001) the percent of the CD31-expressing lymphocytes to 13.21 increased [12.50; 5.76]. Conclusion . The obtained data demonstrate that there is an interrelation between PECAM1 expression as a marker of intercellular interactions and severity of acute pancreatitis.
Highlights
The obtained data demonstrate that the percent of CD31-expressing of lymphocytes depends on weight of acute pancreatitis
The obtained data demonstrate that there is an interrelation between PECAM1 expression as a marker of intercellular interactions and severity of acute pancreatitis
CD31-экспрессирующий лимфоцит (конфокальная микроскопия, оптическое увеличение ×60): слева – окраска ядра DAPI, в центре CD31-экспрессирующий блеббингующий лимфоцит (белая стрелка), рядом – CD31-экспрессирующая микрочастица (красная стрелка), справа – фазово-контрастная микроскопия CD31-expressing lymphocyte (confocal microscopy, optical increase of ×60): left – DAPI nucleus colour, in the center of CD31-expressing blebbinging lymphocyte (white arrow), nearby – CD31-expressing microparticle (red arrow), right – phase-contrast microscopy
Summary
Work has clinical character and is based on the prospective analysis of observation and treatment of 135 patients with acute pancreatitis of various degrees of severity. Patients were divided into three groups, depending on severity of a disease: 1 group — 45 patients with slight acute pancreatitis, the 2nd group — 45 patients with medium-weight acute pancreatitis, the 3rd group — 45 patients with heavy acute pancreatitis. Blood sampling for a research was carried out at receipt prior to therapy. Phenotyping of lymphocytes was carried out by method of an indirect immunofluorescence by means of mouse monoclonal antibodies to the molecules CD31 receptors of lymphocytes
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