Peculiarities of the Expression of immune mediators under aerogenic exposure of aluminum

Abstract

Introduction. Chemical admixtures, including those containing aluminum contaminate the R.F. territory environment. There is a well-proven dependence between hygienic factors and population health. Both facts call for developing scientifically substantiated sanitary recommendations for diagnostics and prevention that should involve applying up-to-date critical technologies, including research on proteins acting as immune modulators. Material and methods. We performed a diagnostic examination and comparatively analyzed the health of children living on territory under the ambient air exposure to aluminum compounds in 78 children who had lived there for not less than four years. The reference group consisted of 20 children who lived in a recreation zone. Immune indices in the examined children were assessed using flow cytometry (Bcl-2, TNFRI), enzyme immunoassay (carcinoembryonic antigen CEA), and allergosorbent (IgG-specific to the aluminum) methods. Genetic indices (TLR4, CPOX, ANKK1) were evaluated by real-time polymerase chain reaction (PCR). We also identified aluminum in ambient air and biological media with mass spectrometry with inductively coupled plasma. Results. We took ambient air exposure to aluminum in 2 reference concentrations. The average aluminum contents in children’s blood were established to be authentically (p < 0.05) higher than reference levels (by 5.5 times) and the same indices in children from the reference group (by 4.5 times). Average aluminum contents in the blood of children from the test group amounted to 0.037 µg/cm3. It was authentically different from the same index in the reference group (0.02 µg/cm3). Simultaneously we revealed that exposed children had polymorph genotypes of proteomic profile genes in blood plasma, namely TLR4 rs1927911, CPOX (rs1131857), ANKK1 rs1800497. Adverse effects of the exposure to aluminum became apparent due to disorders of neural immune regulation as hyperproduction of IgG specific to aluminum and anti-apoptotic transcription protein Bcl-2. It was confirmed by authentic relations between exposure markers and effects that are absent in the reference group as well as by apparent health disorders such as asthenovegetative syndrome. Conclusion. The expression of immune mediators (protein that take part in apoptosis, such as TNFR or anti-apoptotic protein Bcl-2) and related sections in candidate genes (CPOX rs1131857, ANKK1 rs1800497) with polymorphic changes in them can be recommended as indices for determining and preventing the risk of harm to health in conditions of excessive aerogenic contamination with aluminum compounds.