Abstract
Constant human and animal cell lines are widely used by scientists in the study of such essential processes as proliferation, differentiation and malignant transformation. It was shown that the activity of ribosomal genes, determined by Ag-staining, depends on cell proliferation activity (Hall et al., 1988), differentiation level (Reeves et al., 1984) and the degree of malignancy (Crocker et al., 1987). It is essential to study the activity of ribosomal genes in cell lines of different origins by silver staining of metaphase chromosomes and interphase cells by light and electron microscopy. In 30 cell lines (5 normal, 9 leukemic, 3 lymphoma and 13 tumor), the true number of acrocentric chromosomes, including rearranged ones, the number of Ag+ chromosomes, and the number and localization of Ag-NORs on chromosomes were examined. Silver staining of NORs in interphase cells from 5 cell lines (HSL-4, HL-60, U-937, A-431 and 293), which are distinguished by the character of silver staining of metaphase chromosomes, at ultrastructural level was also carried out.
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