Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro

Abstract

Objective — to find out the peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro. Material and methods. The research was conducted in the lab microclonal reproduction of the National Dendropark Sofiyivka of the NAS of Ukraine. The method of microclonal propagation of plants was used, it base on the induction of morphogenic processes that occurred under the influence of phytohormones. The material for research was young, not well-grown shoots taken from 3-year-old plants P. laurocerasus, P. laurocerasus ‘Serbica’, P. laurocerasus ‘Schipcaensis’, P. laurocerasus ‘03.02’ obtained from Novokakhovsky research farm of the Institute of rice of the NAAS of Ukraine. Results. The results of three-year research on the reproduction characteristics of P. laurocerasus, P. laurocerasus ‘Serbica’, P. laurocerasus ‘Schipcaensis’, P. laurocerasus ‘03.02’ in vitro are presented on the use of modified nutrient varieties with different phytohormonal composition. The dependence of the differentiation of explants in these genotypes and processes of morphogenesis, on the exogenous content of phytohormones: 6-benzylaminopurine (6-BAP), indolyl-3-butyric acid (IBA), indolyl-3-acetic acid, 1-naphthylacetic acid (1-NAA) in nutrient media, which contributed to the accelerated appearance of adventitious kidneys. It was found that for P. laurocerasus, P. laurocerasus ‘Schipcaensis’ and P. laurocerasus ‘03.02 the most optimal was the medium Murashige–Scoog (MS-6) to which was added 1.0 mg/l 6-BAP: and the average number of formed shoots respectively was 5.63, 4.66 and 6.61, the average length of shoots was 2.3 cm, and the reproduction coefficient was respectively 6.56, 5.34 and 7.24. For P. laurocerasus ‘Serbica’ the use of the MS-7 medium with the addition of 1.0 mg/l 6-BAP and 0.1 mg/l IBA was effective, which contributed to an average of 4.77 pieces shoots in length 2.09 cm with a multiplication factor of 4.98. Well-developed shoots were transferred to nutrient media for rhizogenesis. The most effective was the nutrient medium of MS with the addition 0.5 and 1.0 mg/l 1-NAA where the rooting of explants respectively was 73.2 and 59.5 %. Conclusions. It has been found that for P. laurocerasus, P. laurocerasus ‘Schipcaensis’ and P. laurocerasus ‘03.02 ‘ the nutrient medium, to which was added 1.0 mg/l 6-BAP, was found to be the most optimal, for P. laurocerasus ‘Serbica’ — nutrient medium with the addition of 1.0 mg/l 6-BAP and 0.1 mg/l IBA. For rhizogenesis the nutrient medium with the addition 0.5 and 1.0 mg/l of 1-NAA was found to be most effective.