Peculiarities of Leukocytic Formula of Peripheral Blood of Green Frogs under Conditions of Anthropogenic Load

Abstract

Changes of the total number of leucocytes and the leucocytic formula of peripheral blood were studied and stability of development was evaluated by the indicator of fluctuating asymmetry of populations of green frogs Rana lessonae Cam. and Rana ridibunda Pall. inhabiting city reservoirs of Nizhni Novgorod and the Nizhegorodskaya Province. The revealed changes in the amphibians white blood depending on the degree of anthropogenic effects in the studied reservoirs can be divided into two types. In the first type (the level of stability of development of populations is 2 scores according to Zakharov) there were revealed neutropenia, increased content of eosinophils, and total leucopenia. In the second type, a more intensive anthropogenic effect and deviation of the organism state from normal conditions, estimated by 3 scores (Zakharov), the leucograms of frogs were characterized by more pronounced negative alterations. Leucocytosis accompanied by neutropenia, eosinophilia, basophilia, and a decrease of the total number of lymphocytes has been established. In the absence of deviations of the stability of development from the conditionally normal state, there was revealed the balance of leucogram indicators, close to norm. An increase of the number of lymphocytes and young forms of neutrophils in the amphibian peripheral blood indicates stimulation of the processes providing the organism protective reaction. Cross-correlation between the integral indicator of stability of development, the content of eosinophils and lymphocytes has been established in individuals from populations of green frogs inhabiting the studied reservoirs.