PECULIARITIES OF ETHANOL INTOXICATION OF ANIMALS AT APPLICATION OF INTERMITTENT HYPOXIC TRAINING AT THE BEGINNING OF ALCOHOL CONSUMPTION

Abstract

Relevance. It was shown a wide therapeutic effects of intermittent hypoxic training (IHT) on the human organism which can increase the organism's resistance to damaging factors by inducing mechanisms of the safety use of energy resources.
 The objective: to investigate the protective effect of IHT applied at the beginning of the 30-day alcohol intoxication of rats on the redox processes and the state of the antioxidant system of blood and liver tissues.
 Materials and methods. The study was performed on white male rats, which were divided into four groups: the first - intact (control); animals of the second group received a 15% ethanol solution for 30 days as the only source of drink; third - animals under IHT; fourth - rats that IHT was applied at the beginning of 30 days alcohol intoxication. The amount of the consumed ethanol was monitored during the experiment. Intermittent hypoxic training was performed in the altitude chamber in the following mode: five 10-min “elevations” to a height of 6 km at a rate of 20 m/sec, intervals between the elevations being 30 min. The activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase), total antioxidant activity (IAOA), as well as the content of lactic and pyruvic acids, malone dialdehyde and diene conjugates were determined. The research materials were homogenates of liver tissues and blood. The process is two-phase, which obviously reflects the stages of alcoholism.
 Results. It was found that alcohol intoxication of animals adapted to IHT is accompanied by an increase in the activity of superoxide dismutase and glutathione peroxidase in the blood and liver tissue against rats that consumed ethanol as the only source of drink. A tendency to increase IAOA and catalase activity against rats that received ethanol for a long time under these conditions was revealed. Analyzing the process of consumption of ethanol solution by animals, we can conclude that animals have a pronounced craving for alcohol in this model of alcoholization. The application of IHT at the beginning of alcohol intoxication as a protective agent delays the phase of formation of a pronounced craving for ethanol and reduces the amount of daily alcohol consumption.
 Conclusions. Thus, sessions of short-term hypoxic effects at the beginning of chronic ethanol consumption, provide an increase in the antioxidant defence of these animals relative to rats with alcohol intoxication.