PECULIARITIES OF BEHAVIORAL REACTIONS OF RATS IN THE BARNES' LABYRINTH AGAINST ALUMINUM CHLORIDE INTOXICATION

Abstract

Relevance. Accumulation of aluminum in brain tissues has a negative effect on the nervous system, which can subsequently lead to memory impairment, emotional instability, decreased motor activity, and therefore to various neurodegenerativediseases. The aim of the work was to evaluate the peculiarities of behavioral reactions in rats against the background of aluminum chloride intoxication and their correction by means of preventive measures. Materials and methods. The experiment was conducted on 32 male white rats weighing from 239 to 268 g, which were divided into four groups: group 1 – intact animals (n = 8); group 2 – injection of 0.5 ml of 12% AlCl3 ×6H2O solution (80 mg Al/kg) (n = 8); group 3 – introduction of a preventive complex against the background of aluminum chloride intoxication: «Lequin» (NPA «Odeska Biotechnology», Ukraine) at a dose of 500 mg/kg, «Gotu Cola» («Nature’s Answer», USA) at a dose of 300 mg/kg and «Sklerosin» (TOV «ECOSVIT OIL», Ukraine) at a dose of 300 mg/kg (n = 8); group 4 – administration of Minerol (NVMP «GOBOR», Ukraine) at a dose of 1000 mg/kg of body weight (n = 8). On the 60th day of aluminum intoxication, the peculiarities of the behavioral reactions of rats of all groups were studied in the Barnes maze using extraneous stimuli (bright light and loud sound). Results and conclusions. A study of the nature of the behavior of rats in the Barnesmaze showed that when performing the task (searching for the «rescue» hole), the animals of each group made mistakes. The highest average number of errors (10) was observed in animals with aluminum intoxication on the 9th day of the experiment (р ≤0.05, Uemp=15.5) compared to control. The average number of errors in this period decreased to 5 and 7, respectively, compared to the previous group, in animals that received the preventive complex and «Minerol» against the background of intoxication. The time to pass the maze (completed task) by rats of the control group compared to the others was the smallest during the entire experiment and from the 5th to the 12th day of the study it further decreased from 48 to 36 s, respectively, and in animals with aluminum intoxication it was the largest. The use of both the preventive complex and «Minerol» reduced the time of passing the maze until the end of the experiment, but these indicators did not reach the values of the control group. Males of the control group chose a direct search strategy in 65-75% of cases. For animals with aluminum intoxication, refusal to perform the task is characteristic both at the beginning of the study and at the end.