Abstract
Hydrolytic activities in liquefying locule tissue of mature-green tomato (Lycopersicon esculentum Mill. `Solar Set') fruit were studied in pursuing the understanding of mechanisms involved in the rheological changes occurring in this tissue. Ethanol-insoluble solids (EIS) were prepared with and without enzyme-inactivating treatment. The release of uronic acids from enzymically active EIS incubated under autolysic conditions was 5-fold higher than recoveries from inactive EIS. Uronic acid release was partially inhibited by 1 mm Hg2+. Cell-free proteins extracts from active EIS exhibited hydrolytic activity against inactive EIS. Pectins released from active EIS showed no evidence of main-chain hydrolysis. Neutral sugars recovered as 80% ethanol-soluble products of autolytic reactions included glc, gal, rha, ara, xyl, and man. Gal was recovered at significant higher levels in autolysates of active EIS. Glycosidases present at high activities in locule tissue included α- and β-galactosidases, β-mannosidase, β-arabinosidase, and β-glucosidase. The results confirm our earlier findings that the metabolism of water, chelator, and alkali-soluble pectins in tomato locule tissue involves deglycosylation with no apparent depolymeriation. These changes alone appear to be inadequate in explaining the unique rheological characteristics in locule gel tissue.
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