Abstract
The pectic enzymes of six strains of pigmented, pectolytic clostridia isolated from potatoes were examined and compared with those strains of Clostridium felsineum and Clostridium aurantibutyricum using cup-plate assays. The organisms could be divided into two groups. Group 1 consisted of the potato isolates and the strain of C. aurantibutyricum; these formed pectic lyase enzymes and pectinesterase but no pectic hydrolase. Group 2 consisted of eight strains of C. felsineum (and one culture labelled Clostridium roseum); these formed pectic lyase and pectic hydrolase but no detectable pectinesterase. Viscometric studies and analyses of degradation products of pectate formed by enzymes of four representative strains from group 1 and two strains from group 2 showed that all these clostridia formed endopectate lyase enzymes; studies of one enzyme preparation showed that the pH optimum was about 9.5. In addition, the group 2 organisms formed endopolygalacturonase and probably also exopolygalacturonase.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
