Peanut yellow spot virus is a member of a new serogroup of Tospovirus genus based on small (S) RNA sequence and organization.

Abstract

Peanut yellow spot virus (PYSV) represents a distinct tospovirus species based on serology and nucleic acid hybridization. THe sequence of the S RNA was 2970 nucleotides with 22 nucleotide long inverted repeats (with three mismatches) at the termini. The coding was ambisense with a long open reading frame (ORF) in each strand. The 5'-large ORF (1,400 nucleotides in the viral sense (v) strand) encoded a protein with a predicted size of 53.2 kDa that was identified as the nonstructural (NSs) protein based on 16-21% sequence identity and 42-48% sequence similarity with other tospoviruses. A 3' ORF (741 nucleotides) in the virus complementary (vc) sense encoded a 28.0 kDa protein that was identified as the nucleocapsid (N) gene based on immuno-blot analysis of the in vitro expressed protein with PYSV polyclonal antiserum. The predicted N protein had 24-28% amino acid sequence identity and 44-51% sequence similarity with the members of other serogroups. In contrast to other tospoviruses, a third ORF (204 nucleotides) occurred in the vc strand, which could encode a protein with a predicted size of 7.5 kDa with two strong hydrophobic regions. The low degree of homology of N and NSs protein sequences with other serogroup members coupled with an additional ORF suggests that PYSV should be classified as a distinct species of the Tospovirus genus. This conclusion also is supported by the absence of serological cross reaction with other serogroups, and biological characteristics including thrips transmission, symptoms and host range.