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Abstract
Germination of peanut seeds in 5 mM N-(phosphonomethyl) glycine isopropylamide, 1, 2-dihydro-3, 6-pyridazinedione (maleic hydrazide), glyphosate, and isopropylamine solutions induced stress response reactions and the release of 24–100 μmol NH4+ per 50 g of seed, with consequent differential suppression of the subunits of glutamate dehydrogenase (GDH). Changes in the GDH isomerization suggested an interaction of the herbicides with the enzyme with consequent increases in the Michaelis constants (Km) from 7.7 mM in the control to 45.0, 50.0 and 77.0 mM NH4+ in the glyphosate, maleic hydrazide, and glyphosate isopropylamide treatments respectively.
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