Abstract
The mechanism of peak broadening in paper chromatography is investigated by comparing the peak widths obtained in chromatography with those caused only by diffusion, for a set of amino acids of widely differing R F values and with eight kinds of Whatman paper. The results show that longitudinal diffusion occurs both in the mobile and the stationary phase in paper chromatography. The mass transfer term is composed of a contribution of unevenness of flow throughout the paper and a contribution of the slowness of attainment of the partition equilibrium between the mobile and the stationary phase. The resistance to attainment of equilibrium resides predominantly in the mobile phase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
