Abstract
ABSTRACT: Peach palm is a domesticated palm commercially important for the production of fruits and hearts of palm. Somatic embryogenesis, an effective technique for mass propagation, was successfully established for this species. Furthermore, a temporary immersion system improved plant regeneration. However, production can be further improved by understanding the peach palm’s growth dynamic and modifications of culture media. The aims of this study were to evaluate the growth of plantlets cultured in different culture media in a temporary immersion system and to correlate the results with nutrient uptake during the growth period. Somatic embryo-derived young plantlets approximately 1 cm in length were cultivated for 12 weeks in a twin flask system containing MS, Y3 or N6 salts, Morel and Wetmore vitamins and 3% sucrose, with a monthly medium refreshment. Growth was measured and mineral analysis of the plantlets was carried out after 12 weeks of culture. The Y3 and MS salts were the most appropriate for the plant growth. Number of roots was 52.52% higher and the root size was 40.42% between the N6 and MS medium and the root number in Y3 medium was 37.74% greater than in MS medium, which is important for post acclimatization survival. K and Na are important elements for peach palm. N is not required at such a high concentration as in Murashige and Skoog formulation. The Chu (N6) medium did not generate high quality plantlets, possibly due to the absence of some micronutrients, like Mo, Cu and Co.
Highlights
Peach palm (Bactris gasipaes Kunth) is a domesticated palm species of tropical America that generates several products of commercial interest, especially hearts of palm
Somatic embryogenesis is a suitable technique for palm species as it allows large-scale production of selected genotypes
The in vitro culture of peach palm was already established by somatic embryogenesis but some bottlenecks showed that the process needs to be improved (STEINMACHER et al 2007a; STEINMACHER et al 2007b; STEINMACHER et al 2007c; MACIEL et al, 2010)
Summary
Peach palm (Bactris gasipaes Kunth) is a domesticated palm species of tropical America that generates several products of commercial interest, especially hearts of palm. Cultivation of this species increased in recent years in Brazil due to its rapid growth, good heart of palm quality and greater market acceptance (SCHROTH et al, 2002; CLEMENT, 2008; GRAEFE et al, 2013). The temporary immersion system enhances the quality of in vitro regeneration protocols and enables larger scale production. A protocol using a temporary immersion system for Bactris gasipaes plantlet regeneration was developed by Steinmacher et al (2011) and this system resulted into more vigorous and taller plantlets when compared with those from a semi-solid culture medium
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
