Abstract

Saponin-like compounds isolated from Pisum sativum were tested for antifungal activity, effect on pea tissue, and effect on chitin and chitosan synthesis in Fusarium solani. Growth of Fusarium solani f. sp. phaseoli and f. sp. pisi macroconidia was inhibited by saponins at concentrations of 150 and 300 μg/ml, respectively. Pod endocarp tissue treated with saponins showed temporary reduction in cell viability (esterase activity); however, there was no significant reduction in resistance to F. solani f. sp. phaseoli, normally incompatible on peas. Macroconidia germinated in the presence of saponin showed decreased incorporation of N-[ 3H]acetylglucosamine into chitin and chitosan at concentrations as low as 32 μg/ml. Thus, a reduction in chitin and chitosan synthesis may be associated with inhibition of fungal growth. Saponins may contribute to the disease resistance of peas

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