Abstract
BackgroundSuppression of PDZRN4 expression in colon cancer tissues may be associated with elevated levels of the microRNA 221 (miR-211). To uncover potential targets for treatment, the present study investigated PDZRN4 in colon cancer development, and explored the role of miR-221-3p in the regulation of PDZRN4.MethodsRNA expression arrays were searched in the NCBI database, and PDZRN4 (PDZ domain containing ring finger 4) was selected as a potential downregulated gene in colon cancer. PDZRN4 mRNA and protein in colon cancer and matched normal tissues were analyzed. The proliferation and dissemination of HCT116 cells overexpressing PDZRN4 was assessed via functional assays. Bioinformatics analysis and luciferase reporter assay were applied to determine the regulatory link between miR-221-3p and PDZRN4 mRNA.ResultsThere was significantly less PDZRN4 mRNA and PDZRN4 protein in colon cancer tissue compared with normal tissues. HCT116 cells overexpressing PDZRN4 were less able to disseminate relative to the control. Expression of PDZRN4 was directly inhibited by miR-221-3p. Knockout of miR-211-3p was associated with attenuated proliferation and dissemination of HCT116 cells.ConclusionsPDZRN4 may function as a tumor suppressor and is downregulated in colon cancer tissues, possibly due to dysregulation via miR-221-3p. This study provides new insight into colon cancer development.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
