PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway.

Haorong He,Qing Zhu,Diyan Li,Menggen Ma,Yao Zhang,Xueke Yu,Huadong Yin

doi:10.3390/ani11041016

Abstract

Simple SummaryPDZ and LIM domain 5 (PDLIM5) can increase C2C12 cell differentiation; however, the role of PDLIM5 in chicken skeletal muscle satellite cells (SMSCs) is unclear. In this study, the effect of PDLIM5 was verified on SMSCs in vitro, and then the molecular mechanism was determined by transcriptome sequencing. We demonstrated that PDLIM5 can positively affect chicken SMSC proliferation and differentiation via the p38-MAPK (mitogen activated kinase-like protein) pathway. These results indicate that PDLIM5 may be involved in chicken skeletal muscle growth and development.Skeletal muscle satellite cell growth and development is a complicated process driven by multiple genes. The PDZ and LIM domain 5 (PDLIM5) gene has been proven to function in C2C12 myoblast differentiation and is involved in the regulation of skeletal muscle development. The role of PDLIM5 in chicken skeletal muscle satellite cells, however, is unclear. In this study, in order to determine whether the PDLIM5 gene has a function in chicken skeletal muscle satellite cells, we examined the changes in proliferation and differentiation of chicken skeletal muscle satellite cells (SMSCs) after interfering and overexpressing PDLIM5 in cells. In addition, the molecular pathways of the PDLIM5 gene regulating SMSC proliferation and differentiation were analyzed by transcriptome sequencing. Our results show that PDLIM5 can promote the proliferation and differentiation of SMSCs; furthermore, through transcriptome sequencing, it can be found that the differential genes are enriched in the MAPK signaling pathway after knocking down PDLIM5. Finally, it was verified that PDLIM5 played an active role in the proliferation and differentiation of chicken SMSCs by activating the p38-MAPK signaling pathway. These results indicate that PDLIM5 may be involved in the growth and development of chicken skeletal muscle.

Highlights

Based on the result of real-time polymerase chain reaction (PCR), we found that the PDZ and LIM domain 5 (PDLIM5) gene was more highly expressed the result of real-time PCR, we found that the PDLIM5 gene was more highly expressed in in chest and leg muscle and the heart among the selected 11 different tissues used (leg chest and leg muscle and the heart among the selected 11 different tissues used and brain) (Figure 1A)
Our results show that myogenic differentiation 1 (MyoD1), and myogenin (MyoG), but these expressive abunPDLIM5 overexpression increased the mRNA expression of myosin heavy chain (MyHC), dances reduced in PDLIM5-knockdown cells (Figure 3A,B)
Our results show that PDLIM5 can promote the proliferation of chicken skeletal muscle satellite cells (SMSCs) by detecting the abundances of proliferation-related genes as well as the viability and proliferation rate of SMSCs after modulating the expression of PDLIM5 in cells

Summary

Introduction

The largest edible part of meat animals is muscle tissue. Muscle is composed of muscle cells ( known as muscle fibers) that can be segmented into skeletal muscle, myocardium, and smooth muscle. Skeletal muscle is regarded as the most abundant, accounting for 45–60% of an adult animal’s body weight [1,2]. The quantity and quality of skeletal muscle directly influence meat production and determine the economic value of animals. Animal skeletal muscle development primarily relies on the activation of skeletal muscle satellite cells (SMSCs) to form new muscle fibers [3,4] while the number of fibers are basically consistent

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