Abstract
Suitable intramuscular fat (IMF) content improves porcine meat quality. The vital genes regulating IMF deposition are necessary for the selection and breeding of an IMF trait. However, the effect and mechanism of PDGFRα on IMF deposition are still unclear. Here, PDGFRα is moderately expressed in porcine longissimus dorsi muscle (LD), whereas it highly expressed in white adipose tissue (WAT). Moreover, PDGFRα-positive cells were located in the gaps of LD fibers which there were IMF adipocytes. Compared with 180-day-old and lean-type pigs, the levels of PDGFRα were much higher in one-day-old and fat-type pigs. Meanwhile the levels of PDGFRα gradually decreased during IMF preadipocyte differentiation. Furthermore, PDGFRα promoted adipogenic differentiation through activating Erk signaling pathway. Based on PDGFRα upstream regulation analysis, we found that the knockdown of FoxO1 repressed lipogenesis by downregulating PDGFRα, and miR-34a inhibited adipogenesis through targeting PDGFRα. Collectively, PDGFRα is a positive regulator of IMF deposition. Therefore, we suggest that PDGFRα is a possible target to improve meat quality.
Highlights
The content of intramuscular fat (IMF) is considered as a crucial indicator of porcine meat quality [1]
Because the different day-old and types of pigs is implicated in meat quality, levels of Platelet-derived growth factor receptor α (PDGFRα) mRNA and protein were detected in longissimus dorsi muscle (LD) using real time qPCR and western blot
Many genes are decreased during preadipocyte differentiation, Consistently, our results indicated that the levels of PDGFRα gradually decreased during IMF preadipocyte differentiation in vitro
Summary
The content of intramuscular fat (IMF) is considered as a crucial indicator of porcine meat quality [1]. The number of IMF progenitors is associated with the IMF deposition [3] It is well-known that adipocytes are derived from mesenchymal stem cells (MSCs), which commit into adipocyte lineage and give rise to preadipocytes [4,5,6]. PDGFRα positive cells are cardiac progenitors, which contribute predominantly to the mesenchymal compartments and vascular [10]. Because PDGFRα expressed in adipogenic stromal cells of adipose tissues, it has been implicated in the adipocyte lineage [11]. Skeletal muscle satellite cells are committed solely to myogenesis [16]. Serve as a positive regulator associated with meat quality for pig selection and breeding of IMF trait InThtherisefsotured,yw, we esueglugceidstaeteddththaet ePfDfecGtFaRnαd croeguuldlatsoerryvme eacshaanpisomsitoifvePDreGgFuRlαatoonr apsisgoIcMiaFted with medaetpqousiatiloitny. fTohrepreigfosree,lewcteiosnugagnedstebdretehdaitnPgDoGfFIRMαFctorualidt. serve as a positive regulator associated with meat quality for pig selection and breeding of IMF trait
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