PD8-07 BONE MARROW-DERIVED CELL SHEET ENGINEERING IMPROVES URINARY FUNCTIONS OF RADIATION-INJURED URINARY BLADDERS IN RAT

Abstract

You have accessJournal of UrologyStem Cell Research1 Apr 2014PD8-07 BONE MARROW-DERIVED CELL SHEET ENGINEERING IMPROVES URINARY FUNCTIONS OF RADIATION-INJURED URINARY BLADDERS IN RAT Tetsuya Imamura, Osamu Ishizuka, Teruyuki Ogawa, Tomonori Minagawa, Hitoshi Yokoyama, Takahiro Yamagishi, Masaki Nakazawa, and Osamu Nishizawa Tetsuya ImamuraTetsuya Imamura More articles by this author , Osamu IshizukaOsamu Ishizuka More articles by this author , Teruyuki OgawaTeruyuki Ogawa More articles by this author , Tomonori MinagawaTomonori Minagawa More articles by this author , Hitoshi YokoyamaHitoshi Yokoyama More articles by this author , Takahiro YamagishiTakahiro Yamagishi More articles by this author , Masaki NakazawaMasaki Nakazawa More articles by this author , and Osamu NishizawaOsamu Nishizawa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.801AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES This study determined if bone marrow-derived cell sheet engineering could improve urinary functions of the radiation-injured urinary bladders in rats. METHODS Twenty female 10-weeks Sprague-Dawley (SD) rats were anesthetized, and then covered with an iron shield except for a 1-cm diameter circle bordering on the pubic bone to allow radiation of the pelvic region containing the urinary bladder. The exposed area was irradiated with 2 Gy once a week for 5 weeks, receiving a total of 10 Gy. Following the last radiation exposure, the rats were maintained for 2 weeks. Bone marrow cells were harvested from both femurs of two male 17-weeks Tg-SD (GFP) rats. The cells were cultured for 7 days, and then the adherent proliferating cells were transferred into temperature-sensitive culture dishes (0.5x106 cells/dish). The transferred bone marrow-derived cells were culture for 2 days. Two days after, by lowering temperature below at 32°C, the cultured cells were harvested as monolayer cell sheet. The cell sheet was transplanted onto the radiated bladder wall (n=10; figure A). The control rats were performed sham operated (n=10). At 4 weeks after transplantation, cystometric investigations were performed. RESULTS At 4 weeks after, the cell sheet-transplanted bladders showed aspects of the recovered urinary walls compared to the control ones (Figure B and C). The basal and micturition pressure did not show differences between both groups. The residual volume of cell sheet-transplanted rats (0.007±0.004 ml) tended to decrease compared to the control rats (0.054±0.029 ml; P=0.058). The voiding interval (6.37±0.71 min), micturition volume (1.18±0.13 ml), and bladder capacity (1.19±0.13 ml) in the cell sheet-transplanted rats were significantly higher than these in control rats (4.08±0.66 min; P<0.05, 0.70±0.03 ml; P<0.05, 0.75±0.13 ml; P<0.05, respectively). CONCLUSIONS The implantation of bone marrow-derived cell sheet recovered aspect of the urinary walls, and improved the voiding interval, micturition volume, and bladder capacity. This study suggested that cell sheet engineering had a great potential to regenerate functional urinary bladders. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e219 Peer Review Report Advertisement Copyright & Permissions© 2014MetricsAuthor Information Tetsuya Imamura More articles by this author Osamu Ishizuka More articles by this author Teruyuki Ogawa More articles by this author Tomonori Minagawa More articles by this author Hitoshi Yokoyama More articles by this author Takahiro Yamagishi More articles by this author Masaki Nakazawa More articles by this author Osamu Nishizawa More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...