PD70-06 THE ROLE OF UROTHELIAL ATP SIGNALING IN MICTURITION REFLEX

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology III1 Apr 2017PD70-06 THE ROLE OF UROTHELIAL ATP SIGNALING IN MICTURITION REFLEX Kentaro Takezawa, Makoto Kondo, Hiroshi Kiuchi, Norichika Ueda, Tetsuji Soda, Shinichiro Fukuhara, Tetsuya Takao, Yasushi Miyagawa, Akira Tsujimura, Kazumasa Matsumoto-Miyai, Yusuke Ishida, Hiromitsu Negoro, Shoichi Shimada, and Norio Nonomura Kentaro TakezawaKentaro Takezawa More articles by this author , Makoto KondoMakoto Kondo More articles by this author , Hiroshi KiuchiHiroshi Kiuchi More articles by this author , Norichika UedaNorichika Ueda More articles by this author , Tetsuji SodaTetsuji Soda More articles by this author , Shinichiro FukuharaShinichiro Fukuhara More articles by this author , Tetsuya TakaoTetsuya Takao More articles by this author , Yasushi MiyagawaYasushi Miyagawa More articles by this author , Akira TsujimuraAkira Tsujimura More articles by this author , Kazumasa Matsumoto-MiyaiKazumasa Matsumoto-Miyai More articles by this author , Yusuke IshidaYusuke Ishida More articles by this author , Hiromitsu NegoroHiromitsu Negoro More articles by this author , Shoichi ShimadaShoichi Shimada More articles by this author , and Norio NonomuraNorio Nonomura More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.3160AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Urothelial ATP signaling has been believed to have an essential role in micturition reflex. To clarify the exact role of urothelial ATP signaling in micturition reflex, we performed detailed in vivo functional analyses in purinergic receptor-deficient mice (Takezawa et al., Sci Rep, 2016). METHODS Male C57BL/6J mice, P2X2-/-, and P2X3-/- mice were used. For the bladder functional analyses, the voided volume per micturition of free-moving mice was assessed by automated voided stain on paper method (Negoro et al., Nat commun, 2012), and the bladder capacity, post-voiding residual volume and intercontraction intervals were evaluated by mouse video-urodynamics testing (Takezawa et al., Am J Physiol Renal Physiol, 2014). (1) Bladder function under normal conditions was examined in wild-type (WT), P2X2-/-, and P2X3-/- mice. (2) Lipopolysaccharide (LPS)-induced changes of bladder function and increases in c-Fos-positive cells in the L6 spinal cord were analyzed in WT, P2X2-/-, and P2X3-/- mice. RESULTS (1) Unexpectedly, a lack of P2X2 or P2X3 receptors did not affect bladder function under normal conditions (Figure 1). (2) In contrast, the LPS-induced bladder hyperactivity and an increase in c-Fos-positive cells in the L6 spinal cord were attenuated in P2X2-/-, and P2X3-/- mice (Figure 2). CONCLUSIONS These findings indicate that urothelial ATP signaling is not essential for normal micturition reflex under physiological conditions, but plays an important role in bladder hyperactivity under pathological conditions. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e1351-e1352 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Kentaro Takezawa More articles by this author Makoto Kondo More articles by this author Hiroshi Kiuchi More articles by this author Norichika Ueda More articles by this author Tetsuji Soda More articles by this author Shinichiro Fukuhara More articles by this author Tetsuya Takao More articles by this author Yasushi Miyagawa More articles by this author Akira Tsujimura More articles by this author Kazumasa Matsumoto-Miyai More articles by this author Yusuke Ishida More articles by this author Hiromitsu Negoro More articles by this author Shoichi Shimada More articles by this author Norio Nonomura More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...