PD56-09 MICROBIOMES IN POST-DIGITAL RECTAL EXAM URINE SAMPLES ARE LINKED TO PROSTATE CANCER RISK

Abstract

You have accessJournal of UrologyProstate Cancer: Detection & Screening VI (PD56)1 Sep 2021PD56-09 MICROBIOMES IN POST-DIGITAL RECTAL EXAM URINE SAMPLES ARE LINKED TO PROSTATE CANCER RISK E David Crawford, Whitney N Stanton, Rick Martin, Caleb D Phillips, Adrie van Bokhoven, M Scott Lucia, Paul Arangua, Francisco G La Rosa, Zachary Grasmick, Gretchen Hoyer, Nelson N Stone, Ryan Terlecki, J Curtis Nickel, and Priya N Werahera E David CrawfordE David Crawford More articles by this author , Whitney N StantonWhitney N Stanton More articles by this author , Rick MartinRick Martin More articles by this author , Caleb D PhillipsCaleb D Phillips More articles by this author , Adrie van BokhovenAdrie van Bokhoven More articles by this author , M Scott LuciaM Scott Lucia More articles by this author , Paul AranguaPaul Arangua More articles by this author , Francisco G La RosaFrancisco G La Rosa More articles by this author , Zachary GrasmickZachary Grasmick More articles by this author , Gretchen HoyerGretchen Hoyer More articles by this author , Nelson N StoneNelson N Stone More articles by this author , Ryan TerleckiRyan Terlecki More articles by this author , J Curtis NickelJ Curtis Nickel More articles by this author , and Priya N WeraheraPriya N Werahera More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002090.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: We investigated the microbiome of post-digital rectal exam (DRE) urine specimens from prostate cancer (PCa) patients and a screening population with a PSA <1.5 ng/mL using PCR (polymerase chain reaction) and next generation sequencing (NGS) techniques. METHODS: 100 post-DRE urine specimens from patients with PCa were compared to 100 specimens from annual Prostate Cancer Awareness Week participants with PSA <1.5 ng/mL (low risk group). Microbial PCR and NGS was performed by MicroGenDX. RESULTS: Analysis of microbiome composition by NGS identified Cutibacterium acnes as significantly more abundant in PCa compared to low risk controls (P < 0. 05) (difference more pronounced in high grade PCa. Finegoldia magna was significantly more abundant in low risk controls (P < 0.05) compared to PCa. PCR differentially identified Klebsiella pneumoniae, Gardnerella vaginalis, Prevotella bivia, and Ureaplasma parvum, in PCa group. PCR detected Escherichia coli, Prevotella bivia, Mycoplasma hominis, Gardnerella vaginalis, and Ureaplasma parvum in the low risk control group. CONCLUSIONS: PCR and NGS analysis of post-DRE urinary microbiome showed detection and abundance differences in PCa compared to low risk controls. Cutibacterium acnes strain Type II identified in PCa in our study has been reported as the most prevalent bacterial species in PCa tissue samples. The presence of Finegoldia magna differentially identified in the group with a low risk of PCa is responsible for the production of equol, a soy metabolite associated with lowering risk of cancer. Our observations indicate the need for corroborative urine, tissue, and semen microbiome studies to identify a possible microbial contribution to prostate cancer risk. Source of Funding: Supported in parts by Schramm Foundation, Bingham Foundation, and Prostate Cancer Biorepository at the University of Colorado Anschutz Medical Campus © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e1007-e1007 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information E David Crawford More articles by this author Whitney N Stanton More articles by this author Rick Martin More articles by this author Caleb D Phillips More articles by this author Adrie van Bokhoven More articles by this author M Scott Lucia More articles by this author Paul Arangua More articles by this author Francisco G La Rosa More articles by this author Zachary Grasmick More articles by this author Gretchen Hoyer More articles by this author Nelson N Stone More articles by this author Ryan Terlecki More articles by this author J Curtis Nickel More articles by this author Priya N Werahera More articles by this author Expand All Advertisement Loading ...