PD49-07 ENGINEERING OF CORPORAL TISSUE CONSTRUCTS USING NON-HUMAN PRIMATE CORPUS CAVERNOSAL SMOOTH MUSCLE AND ENDOTHELIAL CELLS FOR CLINICAL APPLICATIONS

Abstract

You have accessJournal of UrologySexual Function/Dysfunction: Penis/Testis/Urethra: Benign Disease & Malignant Disease I1 Apr 2017PD49-07 ENGINEERING OF CORPORAL TISSUE CONSTRUCTS USING NON-HUMAN PRIMATE CORPUS CAVERNOSAL SMOOTH MUSCLE AND ENDOTHELIAL CELLS FOR CLINICAL APPLICATIONS Joao Zambon, Young-Min Ju, Koudy Williams, Ryan Terlecki, SIta Somara, Alexander Baume, Ashley Dean, John Jackson, Julie Allickson, James Yoo, and Anthony Atala Joao ZambonJoao Zambon More articles by this author , Young-Min JuYoung-Min Ju More articles by this author , Koudy WilliamsKoudy Williams More articles by this author , Ryan TerleckiRyan Terlecki More articles by this author , SIta SomaraSIta Somara More articles by this author , Alexander BaumeAlexander Baume More articles by this author , Ashley DeanAshley Dean More articles by this author , John JacksonJohn Jackson More articles by this author , Julie AllicksonJulie Allickson More articles by this author , James YooJames Yoo More articles by this author , and Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.2231AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Numerous conditions exist, both congenital and acquired, that threaten male sexual health through changes in form and/or function. Efforts in penile reconstruction are often limited by poor availability of functionally intact penile tissue. We have previously demonstrated the ability to reconstruct functional corporal tissue via autologous cell-seeded collagen matrix in a rabbit model. In this study, we investigated the feasibility of engineering corporal constructs using cavernosal smooth muscle (SMCs) and endothelial cells (ECs) from non-human primates (NHPs) seeded onto 3D acellular corporal collagen matrices. METHODS Corpora cavernosa were isolated from NHPs. These specimens were then subjected to an established decellularization process to create acellular corporal collagen matrices. Autologous corporal SMCs and ECs were isolated, expanded in vitro, and seeded onto matrices via a multistep static/dynamic procedure. RESULTS Histologic and immunohistochemical analyses were performed. The corporal construct treated with the TritronX-100 protocol was effectively decellularized based on results of both DAPI (4,6 diamidino 2 phenylindole) staining and DNA assay (< 50 ng dsDNA/mg dry sample weight). Scanning electron microscopy demonstrated highly porous 3D structure and structural integrity. Evenly distributed cellular attachment and phenotype of corporal ECs and SMCs before/after dynamic culture conditioning were evaluated by immunohistochemical staining with anti-von Willebrand factor and anti-alpha smooth muscle actin. CONCLUSIONS We have demonstrated the feasibility of engineering viable and well-organized corpora cavernosa using tissue from NHPs. This represents an important achievement toward clinical utility for humans. Such technology may have application for congenital anomalies, penile cancer, traumatic penile injury, and selected cases of erectile dysfunction and Peyronies disease. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e976 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Joao Zambon More articles by this author Young-Min Ju More articles by this author Koudy Williams More articles by this author Ryan Terlecki More articles by this author SIta Somara More articles by this author Alexander Baume More articles by this author Ashley Dean More articles by this author John Jackson More articles by this author Julie Allickson More articles by this author James Yoo More articles by this author Anthony Atala More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...