PD49-08 INVESTIGATING CORPORAL GENE EXPRESSION PATTERNS IN ISCHEMIC PRIAPISM USING A BERK MOUSE MODEL

Abstract

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology II1 Apr 2018PD49-08 INVESTIGATING CORPORAL GENE EXPRESSION PATTERNS IN ISCHEMIC PRIAPISM USING A BERK MOUSE MODEL Amarnath Mukherjee, Augene Park, Moses Tar, Henny Billet, and Kelvin Davies Amarnath MukherjeeAmarnath Mukherjee More articles by this author , Augene ParkAugene Park More articles by this author , Moses TarMoses Tar More articles by this author , Henny BilletHenny Billet More articles by this author , and Kelvin DaviesKelvin Davies More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2324AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Ischemic priapism is a sustained erection lasting more than 4 hours, and can lead to permanent penile scarring. It is estimated that between 44-90% of priapic episodes lasting more than 24 hours lead to erectile dysfunction (ED). Individuals with hematological disorders, especially those with sickle-cell anemia, are at higher risk of developing priapism. Using sickle transgenic-knockout Berkeley (BERK) mice as our model for sickle-cell anemia, we compared gene expression patterns in the corpora cavernosa between sickle-cell mice and control mice in order to identify genes of interest for future research. METHODS BERK mice and control C57 mice from two different age groups (5-weeks and 12-weeks of age) were sacrificed and their corporal RNA was isolated using Trizol. The 5-week group was considered to be at a “pre-priapic” stage for the BERK mice, and the 12-week group was considered to be at a “priapic” stage for the BERK mice. The RNA was quantified and evaluated for purity. The RNA labeling, microarray hybridization, and quality control were performed by using Affymetrix Clariom S mouse microarray probe sets. The analysis of the genome array data was performed using Affymetrix TAC and EC software following manufacturer's protocol. Changes in gene expression between the BERK and control mice of corresponding age groups were determined and statistically significant results from each age group were then compared to each other to find any genes that were common to both data sets. RESULTS Out of a total of 424 genes identified in the 5-week data set and 437 genes identified in the 12-week data set, 84 genes were found in both sets. Among those common to both sets were genes such as Adh6b (alcohol dehydrogenase 6b), Hspb6 (heat shock protein beta-6), Rxfp1 (relaxin/insulin-like family peptide receptor 1), and Snca (synuclein-alpha). Some genes that appeared in both data sets showed different trends in expression between the two age groups. For example, while Adh6b was upregulated by 3.94-fold in 5-week old BERK mice, it was downregulated by 3.14-fold in 12-week old BERK mice. CONCLUSIONS We have conducted a global screen of progressive changes in gene expression which occur in the sickle cell mice. This screen will identify novel markers of priapism that may be useful targets for preventing or treating priapism in sickle cell patients. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e968 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Amarnath Mukherjee More articles by this author Augene Park More articles by this author Moses Tar More articles by this author Henny Billet More articles by this author Kelvin Davies More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...