PD47-03 EXPRESSION OF CYTOPLASMIC ERβ1 AND NUCLEAR ERβ2 IS ASSOCIATED WITH POOR OUTCOMES FOLLOWING RADICAL PROSTATECTOMY FOR LOCALIZED PROSTATE CANCER

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You have accessJournal of UrologyProstate Cancer: Localized VII1 Apr 2015PD47-03 EXPRESSION OF CYTOPLASMIC ERβ1 AND NUCLEAR ERβ2 IS ASSOCIATED WITH POOR OUTCOMES FOLLOWING RADICAL PROSTATECTOMY FOR LOCALIZED PROSTATE CANCER George R. Schade, Sarah K. Holt, Xiaotun Zhang, Jonathan L. Wright, Shan Shan Zhao, Suzanne Kolb, Hung-Ming Lam, Linda Levin, Yuet-Kin Leung, Shuk-Mei Ho, and Janet Stanford George R. SchadeGeorge R. Schade More articles by this author , Sarah K. HoltSarah K. Holt More articles by this author , Xiaotun ZhangXiaotun Zhang More articles by this author , Jonathan L. WrightJonathan L. Wright More articles by this author , Shan Shan ZhaoShan Shan Zhao More articles by this author , Suzanne KolbSuzanne Kolb More articles by this author , Hung-Ming LamHung-Ming Lam More articles by this author , Linda LevinLinda Levin More articles by this author , Yuet-Kin LeungYuet-Kin Leung More articles by this author , Shuk-Mei HoShuk-Mei Ho More articles by this author , and Janet StanfordJanet Stanford More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2747AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Data regarding the expression of estrogen receptors (ER) and prostate cancer (PCa) outcomes are limited. We evaluated the relationship of expression profiles of ERβ; isoforms and the membrane bound estrogen receptor GPR30 with patient factors and outcomes following radical prostatectomy (RP). METHODS Tissue microarrays constructed from 566 men who underwent RP for localized PCa were analyzed with immunohistochemistry targeting ERβ1, ERβ2, ERβ5 and GPR30. An experienced, blinded pathologist scored receptor distributions and staining intensities. Demographic and clinical data were evaluated with descriptive statistics. Correlations between PCa ERβ; isoform and GPR30 expression and clinicopathologic data were analyzed with χ2 tests. Cox proportional hazards models were used to examine associations between each ER's staining pattern and time to recurrence and prostate cancer-specific mortality (PCSM). Receptor sub-type staining patterns found to have a significant association with recurrence or PCSM were further analyzed using the Kaplan-Meier method. Significance was set at α <0.05, two-tailed test. RESULTS PCa cells had unique staining patterns with ERβ1 demonstrating predominantly nuclear localization, while ERβ2, ERβ5 and GPR30 were predominantly cytoplasmic. Median follow-up among was 10.5 years. After controlling for patient factors, increasing cytoplasmic ERβ1 (cERβ1) (HR 1.53, 95% CI 1.01-2.32, p=0.047) and nuclear ERβ2 (nERβ2) (HR 1.55, 95% CI 1.01-2.38, p=0.043) staining were associated with significantly worse 5-year recurrence-free survival (RFS). Increasing cERβ1 (HR 4.67, 95% CI 1.80 – 12.11, p=0.002) and nERβ2 (HR 2.49, 95% CI 1.08 – 5.80, p=0.033) expression were also associated with significantly increased risk of PCSM. Patients with cERβ1 and nERβ2 co-staining had significantly worse 15-year PCSM vs. patients expressing only cERβ1, only nERβ2, or neither (16.4% vs. 4.3% vs. 0.0% vs 2.0 %, respectively p=0.001) (Figure). CONCLUSIONS Increased cERβ1 and nERβ2 expression are associated with worse PCa-specific outcomes following RP. These findings suggest that evaluating tumor ERβ1 and ERβ2 staining patterns following RP may provide prognostic information © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e962 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information George R. Schade More articles by this author Sarah K. Holt More articles by this author Xiaotun Zhang More articles by this author Jonathan L. Wright More articles by this author Shan Shan Zhao More articles by this author Suzanne Kolb More articles by this author Hung-Ming Lam More articles by this author Linda Levin More articles by this author Yuet-Kin Leung More articles by this author Shuk-Mei Ho More articles by this author Janet Stanford More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...