PD46-02 BLOOD-BASED LIQUID BIOPSY FOR DIAGNOSIS AND SURVEILLANCE OF PRIMARY UPPER TRACT UROTHELIAL CARCINOMA: ROAD TO EMERGING NOVEL BIOMARKER

Abstract

You have accessJournal of UrologyCME1 Apr 2023PD46-02 BLOOD-BASED LIQUID BIOPSY FOR DIAGNOSIS AND SURVEILLANCE OF PRIMARY UPPER TRACT UROTHELIAL CARCINOMA: ROAD TO EMERGING NOVEL BIOMARKER Alireza Ghoreifi, Stephanie Shishido, George Courcoubetis, Salmaan Sayeed, Amy Huang, Andrew Hung, Anne Schuckman, Monish Aron, Mihir Desai, Siamak Daneshmand, Inderbir Gill, Peter Kuhn, Jeremy Mason, and Hooman Djaladat Alireza GhoreifiAlireza Ghoreifi More articles by this author , Stephanie ShishidoStephanie Shishido More articles by this author , George CourcoubetisGeorge Courcoubetis More articles by this author , Salmaan SayeedSalmaan Sayeed More articles by this author , Amy HuangAmy Huang More articles by this author , Andrew HungAndrew Hung More articles by this author , Anne SchuckmanAnne Schuckman More articles by this author , Monish AronMonish Aron More articles by this author , Mihir DesaiMihir Desai More articles by this author , Siamak DaneshmandSiamak Daneshmand More articles by this author , Inderbir GillInderbir Gill More articles by this author , Peter KuhnPeter Kuhn More articles by this author , Jeremy MasonJeremy Mason More articles by this author , and Hooman DjaladatHooman Djaladat More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003359.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Blood-based liquid biopsy has emerged as a novel diagnostic and prognostic biomarker in cancer patients, yet the efficacy of this test in patients with upper tract urothelial carcinoma (UTUC) is unknown. The aim of this study is to explore the feasibility of a liquid biopsy in the diagnosis and surveillance of primary UTUC. METHODS: In this prospective pilot study, under an IRB-approved protocol (#HS-19-00886), blood samples were collected between May 2021 and September 2022 from primary UTUC patients before surgical intervention with curative intent as well as follow-up visits. The samples were analyzed using the third generation comprehensive high-definition single cell assay (HDSCA3.0) to detect rare cells (e.g., circulating tumor cells [CTCs]) and other rare events (e.g., large extracellular vesicles [LEVs]) (Figure 1-A). The findings of pre-surgery liquid biopsies were compared to the blood samples of normal donors (NDs) who had no known pathology. Follow-up samples were compared to the baseline findings. RESULTS: A total of 22 UTUC patients and 50 NDs were included in the analysis. Pathologic staging of UTUC patients revealed 13 non-muscle invasive (<T2) and 9 muscle-invasive (≥T2) tumors. Most cases (17/22) were high-grade. Eight patients received neoadjuvant chemotherapy as well. HDSCA3.0 analysis indicated that rare cells were detectable in the peripheral blood collected from UTUC patients with a high heterogeneity. Total rare events and total rare cells detected in UTUC samples before surgery were significantly higher than that from the NDs. Similar trend was seen in other CTC and rare cell populations, including D|V, D|CK|V|CD, D|V|CD, D, D|CK, and total CK+cells as well as total LEVs and CK+only LEVs (Figure 1-B). Additionally, 11 follow-up samples, collected within a median 8 months following surgery, were available for the analysis that showed significant decrease in the incidence of total LEVs, CK LEVs, and CK|CD LEVs (Figure 1-C). Among these patients, there were 4 bladder recurrences but no distant metastasis. CONCLUSIONS: This study demonstrates promising evidence for the possible clinical utility of the liquid biopsy in UTUC. Rare cells and LEVs may be used in the diagnosis and surveillance of patients with UTUC. Source of Funding: USC Urology Research Council © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e1169 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Alireza Ghoreifi More articles by this author Stephanie Shishido More articles by this author George Courcoubetis More articles by this author Salmaan Sayeed More articles by this author Amy Huang More articles by this author Andrew Hung More articles by this author Anne Schuckman More articles by this author Monish Aron More articles by this author Mihir Desai More articles by this author Siamak Daneshmand More articles by this author Inderbir Gill More articles by this author Peter Kuhn More articles by this author Jeremy Mason More articles by this author Hooman Djaladat More articles by this author Expand All Advertisement PDF downloadLoading ...