PD36-01 THE 5-HT7 RECEPTOR IS UP-REGULATED IN ONUF'S NUCLEUC

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Neurogenic Voiding Dysfunction II1 Apr 2018PD36-01 THE 5-HT7 RECEPTOR IS UP-REGULATED IN ONUF'S NUCLEUC Jianshu Ni, Baojun Gu, nailong cao, and xiaohu wang Jianshu NiJianshu Ni More articles by this author , Baojun GuBaojun Gu More articles by this author , nailong caonailong cao More articles by this author , and xiaohu wangxiaohu wang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1724AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It was previously reported that a 5-HT7 agonist, given intravenously, could enhance external urethral sphincter (EUS) activity and improve voiding efficiency after spinal cord injury (SCI). The present study examined the effect of intrathecal 5-HT7 agonist administration on voiding function, and the change of 5-HT7 receptor expression in lumbosacral cord Onuf's nucleus after SCI. METHODS Thirty-two female SD rats were divided into spinally intact (SI) group and SCI group (n=16 each). Eight weeks after transection, half of the rats underwent continuous cystometry under urethane anesthetization, and the 5-HT7 selective agonist LP44 was given intrathecally. Half of the remaining rats were used for pseudorabies (PRV) retrograde tracing, immunofluorescence (IF) and Western Blot. RESULTS Intrathecally administered LP44 had no effect in spinally intact (SI) rats. In SCI rats, LP44 (1-30 ug/kg) induced significant dose-dependent increases in micturition volume, voiding efficiency, number of high-frequency oscillation (HFO) per micturition, and decrease in residual volume, bladder capacity, and peak bladder pressure. The 5-HT7 receptors antagonist, SB-269970 (10 ug/kg), partially reversed LP44-induced changes. By PRV retrograde tracing and IF, 5-HT7 receptors were demonstrated in L6-S1 spinal cord Onuf's nucleus in both SI and SCI rats, but the expression was significantly stronger in SCI rats. Western blot showed a significant increase of 5-HT7 receptors in ventral L6-S1 spinal cord in SCI rats. CONCLUSIONS A 5-HT7 agonist, given intrathecally, improved voiding efficiency in SCI rats, and the 5-HT7 receptor was significantly up-regulated in lumbosacral cord Onuf's nucleus. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e726 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Jianshu Ni More articles by this author Baojun Gu More articles by this author nailong cao More articles by this author xiaohu wang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...