PD35-01 ARTIFICIAL URINARY SPHINCTER DEVICES HARBOR MICROBIAL BIOFILMS THAT RECONSTITUTE DIFFERENTIALLY IN VITRO BY STRAIN AND MEDICAL DEVICE MATERIAL TYPE

Abstract

You have accessJournal of UrologyCME1 Apr 2023PD35-01 ARTIFICIAL URINARY SPHINCTER DEVICES HARBOR MICROBIAL BIOFILMS THAT RECONSTITUTE DIFFERENTIALLY IN VITRO BY STRAIN AND MEDICAL DEVICE MATERIAL TYPE Glenn Werneburg, Daniel Hettel, Ava Adler, Kenneth Angermeier, Hadley Wood, Sandip Vasavada, Howard Goldman, Raymond Rackley, Bradley Gill, Daniel Shoskes, and Aaron Miller Glenn WerneburgGlenn Werneburg More articles by this author , Daniel HettelDaniel Hettel More articles by this author , Ava AdlerAva Adler More articles by this author , Kenneth AngermeierKenneth Angermeier More articles by this author , Hadley WoodHadley Wood More articles by this author , Sandip VasavadaSandip Vasavada More articles by this author , Howard GoldmanHoward Goldman More articles by this author , Raymond RackleyRaymond Rackley More articles by this author , Bradley GillBradley Gill More articles by this author , Daniel ShoskesDaniel Shoskes More articles by this author , and Aaron MillerAaron Miller More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003333.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The artificial urinary sphincter (AUS) is an effective surgical treatment for stress urinary incontinence, and about 9% of AUS devices require removal due to infection. We sought to determine microbial biofilm and metabolite composition on AUS devices, reconstitute biofilms from microbes isolated from indwelling AUSs in an environment mimicking human tissue, and compare their formation across strains and medical material types. We hypothesized AUSs would harbor unique bacterial and metabolite profiles, and that reconstitution of biofilms in vitro may lead to new strategies to prevent device-associated infection. METHODS: Devices from consented patients scheduled for AUS removal or revision were swabbed for biofilm. Samples and controls were subjected to next-generation sequencing, metabolomics, and culture-based approaches. Bacterial isolates were isolated from devices and grown in a continuous-flow stir-tank bioreactor designed to mimic human tissue with an indwelling medical device present, and biofilm-formation on medical device material types was assessed with plate-count assays and scanning electron microscopy. RESULTS: In the 17 analyzed devices, the most commonly detected phyla were Proteobacteria and Firmicutes. Diethanolamine (local anesthetic precursor), threonine-butyl-ester (precursor for peptide synthesis), 5-aminovaleric acid (bacterial metabolite of lysine; GABA agonist) were the most commonly detected metabolites. Microbes isolated from AUS devices consistently reconstituted biofilm in vitro, and biofilm deposition differed by both strain (Staphylococcus lugdunensis the greatest) and material type (polyurethane the greatest and titanium the least, ****p<0.0001). Micrococcus luteus, shown in the Figure 1, had intermediate biofilm overall, with silicone shown in the top micrograph and polyurethane on the bottom. CONCLUSIONS: AUS devices consistently harbored microbiota in the form of biofilms. Isolated strains reconstituted biofilm in vitro across a library of medical device materials. Biofilm formation differed by both strain and material type. Our methods and results open new avenues to screen for inhibitors of biofilm formation and novel medical device materials resistant to infection. Source of Funding: N/A © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e974 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Glenn Werneburg More articles by this author Daniel Hettel More articles by this author Ava Adler More articles by this author Kenneth Angermeier More articles by this author Hadley Wood More articles by this author Sandip Vasavada More articles by this author Howard Goldman More articles by this author Raymond Rackley More articles by this author Bradley Gill More articles by this author Daniel Shoskes More articles by this author Aaron Miller More articles by this author Expand All Advertisement PDF downloadLoading ...