Abstract
aRNAs from small numbers of PC3 cells spiked in normal blood and recovered using the CellSearch system. RESULTS: RNAs were successfully extracted from as few as 15 CTCs in blood samples. The relative expression levels of reference genes were maintained after RNA amplification. The integrity of the amplified RNA was demonstrated by RT-PCR analysis using primer sets that target the 50-end, middle, and 30-end of reference mRNAs. Besides epithelialand prostate-specific genes, Interleukin-6 was the only gene expressed in all CTCs analyzed. In this pilot study, we found partial concordance between gene expression patterns in CTCs and microdissected prostate cancer tissue from bone metastases of the same patients. CONCLUSIONS: aRNA amplification through in vitro transcription may serve as a useful method for gene analysis in small numbers of CTCs and tumor cells microdissected from BMBxs. To our knowledge, this is the first comparative study of gene expression in CTCs and BMBxs from individual mCRPC patients. Our exploratory study in this small cohort of patients suggests that molecular analysis of CTCs might reveal gene expression patterns that might be missed by analysis of single BMBx sites.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
