Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia I1 Apr 2016PD20-11 THE ROLE OF INDOLEAMINE 2,3-DIOXYGENASE IN EPIDIDYMITIS Ohira Shin, Hara Ryoei, Tone Shigenobu, Fujii Tomohiro, Miyaji Yoshiyuki, Kuribayashi Futoshi, and Nagai Atsushi Ohira ShinOhira Shin More articles by this author , Hara RyoeiHara Ryoei More articles by this author , Tone ShigenobuTone Shigenobu More articles by this author , Fujii TomohiroFujii Tomohiro More articles by this author , Miyaji YoshiyukiMiyaji Yoshiyuki More articles by this author , Kuribayashi FutoshiKuribayashi Futoshi More articles by this author , and Nagai AtsushiNagai Atsushi More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1424AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Indoleamine 2, 3-dioxygenase (IDO) catalyzes the first and rate-limiting step of tryptophan catabolism and has been implicated in immune tolerance. IDO is known to be induced in various tissue during systematic bacterial infection and play a key role in immune response. In our previous research, we elucidated that epididymal IDO expression in the mouse is restricted to the caput region from segments 2 to 5 with peak of expression in segments 3 to 4. However, the role of IDO in epididymitis still remains unclear. We investigated all sorts of cytokines and analyzed specific function of IDO in epididymitis model using IDO knock out mouse in this study. METHODS Twelve weeks old C57BL/6J (wild type: WT and IDO knock-out: IDO KO) male mice were used in this study. Mice were injected with lipopolysaccharide (LPS) 4µg/g (weight) into epididymis on the side of spermatic code. At 7 days after LPS injection, epididymis were removed and examined histologically and biochemically by using cytokine assay (ELISA). Histological finding and expression of cytokines, their experiments were duplicated at least. RESULTS Regarding HE stain, destruction of epididymal ductal structure and invasion of lymphocyte-predominant inflammatory cells were found in epididymitis model of IDO KO mice compared with WT mice. Comprehensive cytokine assay (ELISA) showed that both chemokines (CCL2, CCL3, CCL4, CCL5, CXCL10) and inflammatory promoting cytokines (IL-1ß, IL-2, IL-6, IL-12p70, IL-17, IL-23) were down-regulated in epididymitis model of IDO KO mice compared with WT mice. On the other hand, inflammatory inhibiting cytokines (IL-4, IL-10) were up-regulated. Next, we determined the quantity of IL-6 (ELISA), which is representative marker of inflammatory promoting cytokine. Significant decreased IL-6 expression was statistically observed in epididymitis model of IDO KO mice compared with WT mice (P<0.05). CONCLUSIONS IDO is involved in epididymal inflammation by LPS. IDO might be a novel target for the therapy of the epididymitis in addition to antibodiotics. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e455 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Ohira Shin More articles by this author Hara Ryoei More articles by this author Tone Shigenobu More articles by this author Fujii Tomohiro More articles by this author Miyaji Yoshiyuki More articles by this author Kuribayashi Futoshi More articles by this author Nagai Atsushi More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

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