PD20-09 THE ROLE OF INFLAMMATORY CYTOKINES AND MAPK SIGNALING IN CHRONIC PROSTATITIS/CHRONIC PELVIC PAIN SYNDROME WITH RELATED MENTAL HEALTH DISORDERS

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia I1 Apr 2016PD20-09 THE ROLE OF INFLAMMATORY CYTOKINES AND MAPK SIGNALING IN CHRONIC PROSTATITIS/CHRONIC PELVIC PAIN SYNDROME WITH RELATED MENTAL HEALTH DISORDERS Chao Hu, Hualan Yang, Yanfang Zhao, Xiang Chen, Yinying Dong, Yehao Dong, Jiefeng Cui, Tongyu Zhu, Ping Zheng, Ching-Shwun Lin, and Jican Dai Chao HuChao Hu More articles by this author , Hualan YangHualan Yang More articles by this author , Yanfang ZhaoYanfang Zhao More articles by this author , Xiang ChenXiang Chen More articles by this author , Yinying DongYinying Dong More articles by this author , Yehao DongYehao Dong More articles by this author , Jiefeng CuiJiefeng Cui More articles by this author , Tongyu ZhuTongyu Zhu More articles by this author , Ping ZhengPing Zheng More articles by this author , Ching-Shwun LinChing-Shwun Lin More articles by this author , and Jican DaiJican Dai More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1422AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Mental health disorders (MHD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) have been widely studied. Among these MHD, depression has been identified in 78% of patients with CP/CPPS and even anxious symptoms have been observed in CP/CPPS patients. However, the underlying role of inflammatory cytokines and their associated signaling pathways has not been investigated. Recently, neuroscience research has identified the dysfunction of specific brain areas, such as the basolateral amygdala, nucleus accumbens, hippocampus, and caudate nucleus, are related to anxiety, depression, and spatial and associative memory. Here, we report the potential role of cytokines and associated signaling pathways in CP/CPPS patients with MHD and in a CP/CPPS animal model. METHODS Clinically, CP/CPPS patients (n=810) and control subjects (n=992) were enrolled in this case-control multicenter study with assessment of NIH-CPSI, anxiety and depression. The serum cytokine levels were measured by microarray. Male Sprague-Dawley rats received multiple intracutaneous injections of an immuno-agent along with a pertussis-diphtheria-tetanus triple vaccine for autoimmune CP/CPPS development. The evaluation of rodent animal behavior were performed by elevated plus maze test, open field test, Y maze test and shuttle box passive avoidance test. The levels of cytokines in serum and prostates were detected by ELISA and RT-PCR, and the levels of phospho-MAPK (Erk1/2) in specific brain areas were measured by western blot. All statistical analyses were performed on SAS for Windows 8.0. Statistical significance was considered at a p<0.05 level for all parameters. RESULTS The results revealed that, in CP/CPPS patients with significant MHD, elevated IL-1α, IL-1β, IL-4, IL-13, and TNF-α serum levels were observed. The above five cytokines in CP/CPPS rats were significantly elevated in prostate tissue (p<0.05), and IL-1β levels were elevated in serum and CSF. In behavioral tests, CP/CPPS rats showed anxiety- and depression-like symptoms, and impaired spatial and associative memory performance (p<0.05). In the CP/CPPS group ERK1/2 phosphorylation levels were increased in the amygdala and nucleus accumbens, and decreased in the hippocampus and caudate nucleus. CONCLUSIONS Thus, prostate-derived cytokines, especially IL-1β, cross the blood brain barrier and may lead to enhanced ERK1/2 signaling in several brain areas, possibly underlying induction of CP/CPPS-related MHD. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e454 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Chao Hu More articles by this author Hualan Yang More articles by this author Yanfang Zhao More articles by this author Xiang Chen More articles by this author Yinying Dong More articles by this author Yehao Dong More articles by this author Jiefeng Cui More articles by this author Tongyu Zhu More articles by this author Ping Zheng More articles by this author Ching-Shwun Lin More articles by this author Jican Dai More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...