PD20-04 ROLE OF SPINAL ASTROCYTIC ACTIVATION IN PAIN PATHOGENESIS IN RATS WITH CYCLOPHOSPHAMIDE-INDUCED CYSTITIS

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Interstitial Cystitis1 Apr 2015PD20-04 ROLE OF SPINAL ASTROCYTIC ACTIVATION IN PAIN PATHOGENESIS IN RATS WITH CYCLOPHOSPHAMIDE-INDUCED CYSTITIS Xiangfu Zhou, Bolong Liu, Wenbiao Li, Shaojun Tang, and Minzhi Su Xiangfu ZhouXiangfu Zhou More articles by this author , Bolong LiuBolong Liu More articles by this author , Wenbiao LiWenbiao Li More articles by this author , Shaojun TangShaojun Tang More articles by this author , and Minzhi SuMinzhi Su More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.675AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Constant and recurrent pelvic pain is one of the most important symptoms in the bladder pain syndrome/ interstitial cystitis (BPS/IC). However, the molecular mechanisms underlying BPS/IC remain unclear. Previous studies indicate that BPS/IC pain shares many characteristics of neuropathic pain. Because astrocytic activation plays a critical role in neuropathic pain, we are interested in testing its potential contribution to BPS/IC pain. METHODS Using the rat BPS/IC model induced by cyclophosphamide (CYP), we performed bladder histology to assess the severity of the cystitis with semiquantitative scores and nociceptive behavioral test. Glial fibrillary acidic protein (GFAP) expression in the lumbosacral spinal cord was characterized by immunofluorescent histochemistry and Western blotting. We also determined the effects of intrathecally administrated astrocytic inhibitor L-¦Á-aminoadipate (LAA) on pain and astrocytic activation in the BPS/IC model. RESULTS Compared with control groups, CYP induced clear cystitis (all p values were ©,0.05) and mechanical hypersensitivity in the low abdomen (p©,0.001). Western blot and Immunofluorescent histochemistry indicated that GFAP was significantly up-regulated by cystitis induction (p©,0.05). LAA significantly attenuated CYP-induced astrocytic activation (p©,0.05) and mechanical allodynia (p©,0.05), but without apparent effect on histological damages in bladders (all p values were© ƒ0.05). CONCLUSIONS Our results suggest that spinal astrocyte activation is crucial in the pain pathogenesis of BPS/IC and inhibition of reactive astrocytes could be a novel therapeutic strategy for treating this disorder. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e398-e399 Peer Review Report Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Xiangfu Zhou More articles by this author Bolong Liu More articles by this author Wenbiao Li More articles by this author Shaojun Tang More articles by this author Minzhi Su More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...