PD20-09 ALTERED MICROBIOME IN CHRONIC PELVIC PAIN PATIENTS

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Interstitial Cystitis1 Apr 2015PD20-09 ALTERED MICROBIOME IN CHRONIC PELVIC PAIN PATIENTS David Klumpp, Andrea Braudmeier-Fleming, Ryan Yaggie, Laurie Bachrack, Sarah Flury, Darlene Marko, Matthew Berry, Michael Welge, Colleen Bushell, Bryan White, and Anthony Schaeffer David KlumppDavid Klumpp More articles by this author , Andrea Braudmeier-FlemingAndrea Braudmeier-Fleming More articles by this author , Ryan YaggieRyan Yaggie More articles by this author , Laurie BachrackLaurie Bachrack More articles by this author , Sarah FlurySarah Flury More articles by this author , Darlene MarkoDarlene Marko More articles by this author , Matthew BerryMatthew Berry More articles by this author , Michael WelgeMichael Welge More articles by this author , Colleen BushellColleen Bushell More articles by this author , Bryan WhiteBryan White More articles by this author , and Anthony SchaefferAnthony Schaeffer More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.680AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chronic pelvic pain afflicts millions of patients in the U.S., yet underlying disease mechanisms remain unclear. We recently reported that E. coli strains exhibit diverse pain phenotypes in a murine urinary tract infection model that were subject to modulation via the GI tract, including a chronic pelvic pain phenotype reminiscent of interstitial cystitis/bladder pain syndrome (IC). Thus, we hypothesized that flora of the GI and/or reproductive tracts modulate IC symptoms and characterized the IC microbiome. METHODS The NIDDK-sponsored Multi-Disciplinary Approaches to Chronic Pelvic Pain Network (MAPP) is a comprehensive effort to characterize IC and other chronic pelvic pain syndromes. Female IC patients and controls were recruited into this study from individuals already participating in MAPP at Northwestern University for analyses of fecal and vaginal flora, and a second cohort was recruited that included non-MAPP participants. Stool and vaginal swabs from the introitus, mid-vagina, and posterior fornix were obtained and processed according to the Human Microbiome Project Manual of Procedures. Duplicate samples were cultured under anaerobic conditions. Purified DNA samples were subjected to 16S rDNA sequence and metagenome sequence analyses. RESULTS Random forest analyses identified statistically significant features that discriminated IC patients from controls. Consistent with differences in microbiomes, oral gavage of anaerobic cultures from IC patients and controls into mice differentially modulated pelvic pain. CONCLUSIONS These preliminary studies suggest that chronic pelvic pain is associated with an altered fecal microbiome in IC patients. Moreover, specific taxa may modulate IC symptoms and represent novel biomarkers for diagnosis. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e401 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information David Klumpp More articles by this author Andrea Braudmeier-Fleming More articles by this author Ryan Yaggie More articles by this author Laurie Bachrack More articles by this author Sarah Flury More articles by this author Darlene Marko More articles by this author Matthew Berry More articles by this author Michael Welge More articles by this author Colleen Bushell More articles by this author Bryan White More articles by this author Anthony Schaeffer More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...