PD19-03 BLADDER MICROBIOTA DIFFER IN MEN AND WOMEN WITH AND WITHOUT LOWER URINARY TRACT SYMPTOMS

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology I1 Apr 2018PD19-03 BLADDER MICROBIOTA DIFFER IN MEN AND WOMEN WITH AND WITHOUT LOWER URINARY TRACT SYMPTOMS Petar Bajic, Michelle Van Kuiken, Bethany Burge, Eric Kirshenbaum, Alan Wolfe, Kristin Baldea, Larissa Bresler, and Ahmer Farooq Petar BajicPetar Bajic More articles by this author , Michelle Van KuikenMichelle Van Kuiken More articles by this author , Bethany BurgeBethany Burge More articles by this author , Eric KirshenbaumEric Kirshenbaum More articles by this author , Alan WolfeAlan Wolfe More articles by this author , Kristin BaldeaKristin Baldea More articles by this author , Larissa BreslerLarissa Bresler More articles by this author , and Ahmer FarooqAhmer Farooq More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.993AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bacteria have been identified in urine deemed sterile by standard culture and have been shown to influence lower urinary tract symptoms (LUTS). We compare male and female bladder microbiota in patients with and without LUTS. METHODS Catheterized urine specimens (CUS) were collected from 49 adult men: 29 with benign prostatic hyperplasia (BPH)/LUTS, and 20 asymptomatic males (ASM). BPH patient samples were compared to 55 CUS from age-matched women with overactive bladder (OAB). We also compared the 20 ASM to 107 CUS from age-matched asymptomatic females (ASF). Bacterial growth was assessed using Expanded Quantitative Urine Culture (EQUC), which utilizes 100x more urine, additional media and environmental conditions, with double the incubation period of standard culture. Colonies grown were identified by mass spectrometry. EQUC growth and demographics were compared using chi-squared tests and Welch t-tests. Odds ratios for symptoms associated with EQUC growth were reported from multivariable logistic regression models controlling for age and hypertension (HTN) in men and age, HTN and BMI in women. RESULTS EQUC positivity was seen in 64.6% (135/209) of women compared to 32.7% (16/49) of men (p < 0.001). On multivariate analysis, men with BPH/LUTS were EQUC positive more often than ASM with an OR of 6.9 (95% CI 1.1-42.8, p = 0.038) and women with OAB were EQUC positive more often than ASF with an OR of 3.9 (95% CI 1.6-9.5, p = 0.003). OAB patients showed mixed microbial composition in 23.6% (13/55) of CUS, while BPH/LUTS patients were all dominated by a single genus (p = 0.003). Lactobacillus dominated in 21.6% (35/162) of women, but was absent in men (p < 0.001). CONCLUSIONS Male bladders are EQUC positive less often than their female counterparts, both in the presence and absence of LUTS. Males and females with LUTS are more likely to have bladder microbiota identifiable by EQUC than their asymptomatic counterparts. Mixed microbial composition is more common in symptomatic than asymptomatic female patients, while male patients are typically dominated by a single genus. Lactobacillus dominance was only identified in female patients. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e395-e396 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Petar Bajic More articles by this author Michelle Van Kuiken More articles by this author Bethany Burge More articles by this author Eric Kirshenbaum More articles by this author Alan Wolfe More articles by this author Kristin Baldea More articles by this author Larissa Bresler More articles by this author Ahmer Farooq More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...