PD11-02 PROSTATE INFLAMMATION AND COLLAGEN CONTENT ARE INVERSELY CORRELATED IN HUMAN BENIGN PROSTATIC HYPERPLASIA

Abstract

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research & Pathophysiology (PD11)1 Sep 2021PD11-02 PROSTATE INFLAMMATION AND COLLAGEN CONTENT ARE INVERSELY CORRELATED IN HUMAN BENIGN PROSTATIC HYPERPLASIA Emily Serrell, Andrew Schneider, Matthew Grimes, Sijan Wang, and Wade Bushman Emily SerrellEmily Serrell More articles by this author , Andrew SchneiderAndrew Schneider More articles by this author , Matthew GrimesMatthew Grimes More articles by this author , Sijan WangSijan Wang More articles by this author , and Wade BushmanWade Bushman More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001986.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Prostate inflammation and fibrosis have been implicated in the development of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Mouse studies have shown prostate inflammation to produce tissue hyperplasia and an increase in prostatic collagen content (fibrosis). Here we examined the connections of inflammation, age, prostate volume (PV) and collagen content in human BPH surgical specimens. METHODS: Prostate tissue specimens were collected from patients treated by surgery for BPH/LUTS from 2005-2016. Patients were excluded if pathologic examination revealed >5% prostate cancer. PV was determined by transrectal ultrasound or pelvic CT scan. Collagen content was assessed by picrosirius red staining and digitally quantified. Inflammation was assessed by immunostaining with the pan-leukocyte marker CD45 and scored manually by a blinded observer. Associations between PV, age, collagen, and inflammation were evaluated by scatterplot analysis. RESULTS: In 53 men (mean age 65.1 (47-88)), the average PV was 65.5cc (47-88). Collagen content was positively correlated with age (0.34, p=0.01) but was negatively correlated with total inflammation score (r=-0.28, p=0.04). The correlations between increasing PV and both inflammation and collagen content were bimodal with a significant change point at 70 grams (p=0.002). For smaller prostates, inflammation increased (0.49, p=0.002) and collagen decreased (-0.35, p=0.03). For larger prostates, inflammation decreased (-0.29, p=0.30) and collagen increased (0.17, p=0.23). CONCLUSIONS: Our data reveal that age is a strong determinant of collagen content in BPH. In contrast to studies performed in mice, we found an inverse relationship between inflammation and collagen content. This difference may reflect different net effects of inflammation induced collagen degradation and synthesis in the dense stroma of the human prostate versus the more delicate stroma of the mouse. Finally, we found that small and larger prostates have a fundamentally different relationship to inflammation and collagen content, suggesting distinctive molecular or genetic mechanisms for prostate growth and inflammation. Source of Funding: VA Merit Award. I01 BX003454-01 © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e197-e198 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Emily Serrell More articles by this author Andrew Schneider More articles by this author Matthew Grimes More articles by this author Sijan Wang More articles by this author Wade Bushman More articles by this author Expand All Advertisement Loading ...