PD09-12 MULTIPHOTON MICROSCOPY: A NEW INTRA-OPERATIVE TOOL FOR TURBT DIAGNOSIS

Abstract

You have accessJournal of UrologyBladder Cancer: Non-invasive I (PD09)1 Sep 2021PD09-12 MULTIPHOTON MICROSCOPY: A NEW INTRA-OPERATIVE TOOL FOR TURBT DIAGNOSIS Patrick Julien Treacy, Matthieu Durand, Z. S. Dovey, I. Pavlova, J. Sfakianos, R. Mehrazin, U. G. Falagario, E. Wajswol, V. M. Padilla, Imad Bentellis, Flora Barthe, Daniel Chevallier, R. Cabal, J. Walz, N. Tzavaras, A. K. Tewari, and P. Wiklund Patrick Julien TreacyPatrick Julien Treacy More articles by this author , Matthieu DurandMatthieu Durand More articles by this author , Z. S. DoveyZ. S. Dovey More articles by this author , I. PavlovaI. Pavlova More articles by this author , J. SfakianosJ. Sfakianos More articles by this author , R. MehrazinR. Mehrazin More articles by this author , U. G. FalagarioU. G. Falagario More articles by this author , E. WajswolE. Wajswol More articles by this author , V. M. PadillaV. M. Padilla More articles by this author , Imad BentellisImad Bentellis More articles by this author , Flora BartheFlora Barthe More articles by this author , Daniel ChevallierDaniel Chevallier More articles by this author , R. CabalR. Cabal More articles by this author , J. WalzJ. Walz More articles by this author , N. TzavarasN. Tzavaras More articles by this author , A. K. TewariA. K. Tewari More articles by this author , and P. WiklundP. Wiklund More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001977.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Test the capacity of multiphoton microscopy (MPM) in bladder Muscularis Propria (MP) detection of patients undergoing TURBT (Trans-Urethral Resection of Bladder Tumor), as a real-time imaging tool. METHODS: Prospective feasibility study, all patients were consented (IRB: GCO#14-0318/HSM14-0024). Cold cup biopsies were taken from the bladder resection bed during TURBT. Tissue was stored in PBS solution with fluorescent dyes (orange acridine and rhodamine). MPM Imaging of the tissue was done first, with z stack images for 3D imaging (Figure 1). Procedure time, MPM imaging depth, After MPM imaging, tissue was fixed in formalin and sent to biorepository for standard histopathology, and correlation analysis was performed. RESULTS: 10 patients were included (Table 1). Tissue was properly imaged for all patients, although 3 of them had artifacts, and we detected 5 times MP on MPM imaging. MPM average procedure time from biopsy to formalin fixation was 16.2 minutes (12-20 min). On final pathology, resection depth was correctly performed for only 4 patients out of 10 (resection including MP)( Figure 2). We found an 80 % sensitivity on resection depth between first layer identified on MPM and final pathology (8/10 patients), and 100% correlation with final histopathology in specimens for the assessment of MP. CONCLUSIONS: This study supports the use of MPM as a novel intra operative tool which can evaluate cold cup biopsies during TURBT for resection depth, and potentially avoid unnecessary second look procedure. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e124-e125 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Patrick Julien Treacy More articles by this author Matthieu Durand More articles by this author Z. S. Dovey More articles by this author I. Pavlova More articles by this author J. Sfakianos More articles by this author R. Mehrazin More articles by this author U. G. Falagario More articles by this author E. Wajswol More articles by this author V. M. Padilla More articles by this author Imad Bentellis More articles by this author Flora Barthe More articles by this author Daniel Chevallier More articles by this author R. Cabal More articles by this author J. Walz More articles by this author N. Tzavaras More articles by this author A. K. Tewari More articles by this author P. Wiklund More articles by this author Expand All Advertisement Loading ...