PD08-11 REAL-TIME MONITORING OF RADIOFREQUENCY ABLATION RANGE OF RENAL TUMORS BY THERMOSENSITIVE LIPID NANOPARTICLES

Abstract

You have accessJournal of UrologyKidney Cancer: Localized: Ablative Therapy (PD08)1 Apr 2020PD08-11 REAL-TIME MONITORING OF RADIOFREQUENCY ABLATION RANGE OF RENAL TUMORS BY THERMOSENSITIVE LIPID NANOPARTICLES Linfang Yao*, Guanchen Zhu, Wenmin Cao, Wei Wang, Wei Chen, Ming Chen, Xingqun Zhao, and Hongqian Guo Linfang Yao*Linfang Yao* More articles by this author , Guanchen ZhuGuanchen Zhu More articles by this author , Wenmin CaoWenmin Cao More articles by this author , Wei WangWei Wang More articles by this author , Wei ChenWei Chen More articles by this author , Ming ChenMing Chen More articles by this author , Xingqun ZhaoXingqun Zhao More articles by this author , and Hongqian GuoHongqian Guo More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000835.011AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To explore the feasibility of assessing intraoperative radiofrequency ablation coverage by using novel thermosensitive lipid nanoparticles contained with liquid perfluorohexane. METHODS: In this experiment, a new kind of thermosensitive lipid nanoparticle contained with liquid perfluorohexane was prepared by rotating evaporation and acoustic resonance method, which could be changed from liquid to gas quickly at 58-60 °C. Thermal phase transition of nanoparticles was observed in vitro water capsule heating experiment and agar-agar gel model radiofrequency ablation experiment. In vivo rabbit kidney VX2 tumor model was used for ultrasound imaging with the nanoparticles. Radiofrequency ablation alone and radiofrequency ablation combined with this thermosensitive nanoparticles were performed respectively. The formation of gasification area was observed with ultrasonic measuring system (Flex Focus 800 (BK Medical). The boundary temperature of the gasification zone was detected by the temperature measuring system(NATIONAL INSTRUMENTS Company, USA). The size of gasification area and gross pathology were measured, and their correlation was analyzed. RESULTS: Through in vitro characterization, the size of this nanoparticles was confirmed to be about 300nm. In vitro ultrasound experiments showed that the echo of thermosensitive lipid nanoparticles was significantly enhanced compared with that before heating. Radiofrequency ablation of rabbit renal VX2 tumor in vivo showed that thermosensitive lipid nanoparticles made the gasification zone generated by radiofrequency ablation more obvious, and the boundary temperature of the gasification zone was about 60?. The gasification zone is larger than the radiofrequency ablation range, and highly correlated with gross pathologic necrosis zone. CONCLUSIONS: Thermosensitive lipid nanoparticles contained with liquid perfluorohexane have the potential of thermosensitive phase transition. It is expected to be a new method for intraoperative real-time monitoring and evaluation of radiofrequency ablation boundaries. Source of Funding: This work was supported by the National Natural Science Foundation of China (Grant No. 81972388, 81772710) , Nanjing health science and technology development special fund project?YKK18076?,Jiangsu postdoctoral research fund?1701023B?, the Project of Invigorating Health Care through Science, Technology and Education, Jiangsu Provincial Key Medical Discipline (Laboratory) (ZDXKB2016014). © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e172-e173 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Linfang Yao* More articles by this author Guanchen Zhu More articles by this author Wenmin Cao More articles by this author Wei Wang More articles by this author Wei Chen More articles by this author Ming Chen More articles by this author Xingqun Zhao More articles by this author Hongqian Guo More articles by this author Expand All Advertisement PDF downloadLoading ...