PD08-03 EFFECTS OF DIFFERENT RADIOFREQUENCY ABLATION MODES ON NORMAL RENAL TISSUE AROUND THE ABLATION ZONE

Abstract

You have accessJournal of UrologyKidney Cancer: Localized: Ablative Therapy (PD08)1 Apr 2020PD08-03 EFFECTS OF DIFFERENT RADIOFREQUENCY ABLATION MODES ON NORMAL RENAL TISSUE AROUND THE ABLATION ZONE Linfang Yao*, Wenmin Cao, Wei Wang, Wei Chen, Jiong Shi, Xingqun Zhao, and Hongqian Guo Linfang Yao*Linfang Yao* More articles by this author , Wenmin CaoWenmin Cao More articles by this author , Wei WangWei Wang More articles by this author , Wei ChenWei Chen More articles by this author , Jiong ShiJiong Shi More articles by this author , Xingqun ZhaoXingqun Zhao More articles by this author , and Hongqian GuoHongqian Guo More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000835.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To explore the effects of radiofrequency ablation "slow" or "fast" mode on normal renal tissue around the ablation zone. METHODS: The animal model of rabbit renal VX2 tumor was established. Radiofrequency ablation of rabbit renal VX2 tumors was performed in a "slow" or "fast" mode. The thermocouple temperature measurement system(NATIONAL INSTRUMENTS Company, USA) monitored the temperature changes of the ablation zone and the renal tissue around the ablation zone during the radiofrequency ablation. The formation and range of radiofrequency ablation were monitored in real time by B-ultrasound imaging system (Flex Focus 800 (BK Medical).Conventional pathological hematoxylin-eosin staining was used to observe the necrosis. Cell necrosis and apoptosis were detected by trypan blue staining and TUNEL(terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling). The change of cell microstructure was examined under a transmission electron microscope. RESULTS: Radiofrequency ablation of rabbit renal VX2 tumors in either "slow" or "fast" mode formed an ideal range of ablation zone to cover the tumor.The average temperature within the ablation area reached 80 degree centigrade and a significant ablation zone could be formed. But the "fast" mode of radiofrequency ablation often leaded to a temperature of more than 60 degree centigrade in normal kidney tissue around the ablation zone. While under a "slow" mode, the temperature of normal surrounding kidney tissue was about 40 degree centigrade. The "fast" mode of radiofrequency ablation could easily lead to damage to normal renal tissue around the ablation zone followed with degeneration and necrosis. Trypan blue staining was positive in the damaged tissue cells. Apoptosis index and mitochondrial injury were increased. While the "slow" mode of radiofrequency ablation did not cause significant damage to normal renal tissue surrounding the ablation zone. CONCLUSIONS: Radiofrequency ablation of renal tumors should be performed in a "slow" mode to minimize damage to normal renal tissue around the ablation zone. Source of Funding: This work was supported by the National Natural Science Foundation of China (Grant No. 81972388, 81772710) , Nanjing health science and technology development special fund project (YKK18076), Jiangsu postdoctoral research fund (1701023B), the Project of Invigorating Health Care through Science, Technology and Education, Jiangsu Provincial Key Medical Discipline (Laboratory) (ZDXKB2016014). © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e168-e168 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Linfang Yao* More articles by this author Wenmin Cao More articles by this author Wei Wang More articles by this author Wei Chen More articles by this author Jiong Shi More articles by this author Xingqun Zhao More articles by this author Hongqian Guo More articles by this author Expand All Advertisement PDF downloadLoading ...