PD07-12 POST-THAW OUTCOMES OF RARE OR VERY LOW CONCENTRATIONS OF CRYOPRESERVED SPERM

Abstract

You have accessJournal of UrologyInfertility: Therapy II1 Apr 2016PD07-12 POST-THAW OUTCOMES OF RARE OR VERY LOW CONCENTRATIONS OF CRYOPRESERVED SPERM Nikita Abhyankar, Martin Kathrins, Juergen Liebermann, Meike Uhler, Yoshitomo Kobori, Gail Prins, and Craig Niederberger Nikita AbhyankarNikita Abhyankar More articles by this author , Martin KathrinsMartin Kathrins More articles by this author , Juergen LiebermannJuergen Liebermann More articles by this author , Meike UhlerMeike Uhler More articles by this author , Yoshitomo KoboriYoshitomo Kobori More articles by this author , Gail PrinsGail Prins More articles by this author , and Craig NiederbergerCraig Niederberger More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2815AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Our aim was to examine instances in which no sperm could be identified after thaw among cryopreserved samples of rare or very low counts of sperm. METHODS We performed a retrospective review of our institutional fertility clinic database from 2010 until 2015. We identified couples that underwent intracytoplasmic sperm injection (ICSI) with either ejaculated or testicular cryopreserved-thawed sperm. Inclusion criteria was limited to men with ejaculated cryptozoospermia and severe oligozoospermia (< 100,000 total sperm per specimen) or men with azoospermia due to spermatogenic defects who underwent microsurgical testicular sperm extraction (mTESE) with similarly low pre-cryopreservation total sperm counts. Sperm were cryopreserved in 1 mL vials using liquid nitrogen. Pre-cryopreservation specimens were categorized as rare sperm only (Group 1) or less than 100,000 total sperm (Group 2). The primary outcome was the availability of any post-thaw sperm available for use at the time of ICSI. The relationship between successful post-thaw sperm retrieval and pre-cyropreservation parameters was examined using Chi-square statistical analysis for ordinal variables. RESULTS We identified 34 couples who met inclusion criteria and with sufficient follow-up data, encompassing 49 ICSI cycles (Table 1). There were three ICSI cycles (6.1% of all ICSI cycles) among three different couples in which no sperm were able to be identified post-thaw. All three instances (n=1 ejaculated source, n=2 testicular source) were noted to have pre-thaw parameters of rare sperm only (Group 1) (8.6% of ICSI cycles utilizing cryopreserved rare sperm only). Chi-square analysis of the ability to locate sperm post-thaw and pre-cryopreservation total sperm count revealed a significant association (Pearson X2=49.0, df=2, p=<0.001). CONCLUSIONS Failure to retrieve any sperm after thawing of rare or very low quantities of cryopreserved sperm is an infrequent event and limited to those patients with rare quantities of sperm prior to cryopreservation. Although this study is limited in size, the results may influence recommendation to have back-up donor sperm available or to consider use of fresh sperm in further mTESE procedures for future ICSI cycles among men with cryopreserved rare sperm. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e227-e228 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Nikita Abhyankar More articles by this author Martin Kathrins More articles by this author Juergen Liebermann More articles by this author Meike Uhler More articles by this author Yoshitomo Kobori More articles by this author Gail Prins More articles by this author Craig Niederberger More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...