PD05-11 SERUM ANANDAMIDE AND LIPIDS ASSOCIATED WITH LINOLEIC ACID CAN DISTINGUISH INTERSTITIAL CYSTITIS AND BLADDER PAIN SYNDROME FROM OVERACTIVE BLADDER

Abstract

You have accessJournal of UrologyCME1 Apr 2023PD05-11 SERUM ANANDAMIDE AND LIPIDS ASSOCIATED WITH LINOLEIC ACID CAN DISTINGUISH INTERSTITIAL CYSTITIS AND BLADDER PAIN SYNDROME FROM OVERACTIVE BLADDER Kazumasa Torimoto, Tomohiro Ueda, Daisuke Gotoh, Kuniyuki Kano, Makito Miyake, Yasushi Nakai, Shunta Hori, Yosuke Morizawa, Kenta Onishi, Takuto Shimizu, Mitsuru Tomizawa, Junken Aoki, and Kiyohide Fujimoto Kazumasa TorimotoKazumasa Torimoto More articles by this author , Tomohiro UedaTomohiro Ueda More articles by this author , Daisuke GotohDaisuke Gotoh More articles by this author , Kuniyuki KanoKuniyuki Kano More articles by this author , Makito MiyakeMakito Miyake More articles by this author , Yasushi NakaiYasushi Nakai More articles by this author , Shunta HoriShunta Hori More articles by this author , Yosuke MorizawaYosuke Morizawa More articles by this author , Kenta OnishiKenta Onishi More articles by this author , Takuto ShimizuTakuto Shimizu More articles by this author , Mitsuru TomizawaMitsuru Tomizawa More articles by this author , Junken AokiJunken Aoki More articles by this author , and Kiyohide FujimotoKiyohide Fujimoto More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003229.11AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Interstitial cystitis/bladder pain syndrome (IC/BPS) is challenging to diagnose because it relies on subjective symptoms and empirical cystoscopic findings. We performed metabolomics on the serum of participants with IC/BPS and without lower urinary tract symptoms (LUTS), and identified serum 1-linoleoyl-glycero-phosphocholine as a candidate diagnostic biomarker of Hunner type IC (HIC). However, in a subsequent study, the serum levels were found to be lower in HIC, BPS, and overactive bladder (OAB) than in those without LUTS. Therefore, we performed the second metabolomics on the serum of these four groups to identify better biomarkers. METHODS: We enrolled participants with HIC (n=20), BPS (n=20), OAB (n=20), and without LUTS (n=15). Patients with BPS showed mucosal bleeding after distension. Metabolomics was performed on 323 metabolites in serum using liquid chromatography-mass spectrometry. RESULTS: Partial least squares discriminant analysis revealed that the relative areas including anandamide, acylcarnitine (18:2) and linoleoyl ethanolamide, arachidonic acid were smaller in the groups with HIC or BPS than those with OAB or without LUTS. The difference in the relative areas of anandamide was remarkable, and the ability to discriminate IC/BPS exhibited an area under the curve of 0.9321, a sensitivity of 80.00%, and a specificity of 88.57%. CONCLUSIONS: Serum anandamide may be the best candidate as a diagnostic biomarker for IC/BPS. Low serum anandamide levels might be associated with the initiation of pain and inflammation, and may reflect the pathology of IC/BPS. The major candidates are linoleic acid metabolites or have linoleic acid in their side chains. This suggests the involvement of abnormal metabolism of linoleic acid in the pathogenesis of IC/BPS. Source of Funding: None © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e153 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kazumasa Torimoto More articles by this author Tomohiro Ueda More articles by this author Daisuke Gotoh More articles by this author Kuniyuki Kano More articles by this author Makito Miyake More articles by this author Yasushi Nakai More articles by this author Shunta Hori More articles by this author Yosuke Morizawa More articles by this author Kenta Onishi More articles by this author Takuto Shimizu More articles by this author Mitsuru Tomizawa More articles by this author Junken Aoki More articles by this author Kiyohide Fujimoto More articles by this author Expand All Advertisement PDF downloadLoading ...