PD05-10 ICSI OUTCOME IN PATIENTS WITH HIGH DNA FRAGMENTATION: TESTICULAR VS EJACULATED SPERM: QATAR EXPERIENCE

Abstract

You have accessJournal of UrologyInfertility: Therapy I1 Apr 2016PD05-10 ICSI OUTCOME IN PATIENTS WITH HIGH DNA FRAGMENTATION: TESTICULAR VS EJACULATED SPERM: QATAR EXPERIENCE Ahmad Hassan AlMalki, Haitham T ElBardisi, Sami S AlSaid, Hassan Burjaq, Moza AlBadr, and Mohamad M Arafa Ahmad Hassan AlMalkiAhmad Hassan AlMalki More articles by this author , Haitham T ElBardisiHaitham T ElBardisi More articles by this author , Sami S AlSaidSami S AlSaid More articles by this author , Hassan BurjaqHassan Burjaq More articles by this author , Moza AlBadrMoza AlBadr More articles by this author , and Mohamad M ArafaMohamad M Arafa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2617AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Sperm DNA Fragmentation Index (DFI) has emerged as an important biomarker in the assessment of male fertility potential. There are many controversies involving the routine use of DFI in clinical evaluation of infertile male. Another controversy is using testicular sperm instead of ejaculated sperm aiming to improve assisted reproductive techniques outcomes. The aim of the present study was to evaluate Intra Cytoplasmic Sperm Injection (ICSI) outcomes in male patients with high DFI using Testicular Vs. Ejaculated Sperm. METHODS This is a prospective study of 38 men with high DFI not responding to treatment who were scheduled for ICSI between January 2014 to January 2015. Inclusion criteria were (1) High DFI (= 30% using HaloSperm) not responding to treatment (2) Previous ICSI trial using ejaculated sperm. Couples with female factor infertility were excluded. Routine semen analysis was done including sperm DFI. ICSI data of the previous cycle where ejaculated sperm were used for injection was documented. On the day of ICSI, retrieved oocytes were fertilized using testicular sperms obtained by TESA. Comparison of outcomes measures (oocytes fertilized, quality of embryos, clinical pregnancy rate and live birth rate) of the two fertilization techniques were documented. RESULTS Two patients were excluded from the study due to presence of female factor infertility. Mean male age was 38±12, while mean female age was 31±5. Semen Fluid Analysis (SFA) characteristics were as follow: Count 16±20; Motility (progressive) 19±20; Abnormal forms 86±16; Round cells (0.04±0.11)X10^6 and DFI was 56±15. Overall, there was no difference in the fertilization rate and embryo grading using ejaculate and testicular sperms (46.4% vs. 47.8%, 50.2% vs. 53.4% respectively). However, clinical pregnancy was significantly higher in TESA group compared to ejaculated group (38,89% (14 of 36) vs 13.8% (5 of 36)). Also, 17 live births were documented in TESA group and only 3 live births were documents in ejaculate group (p<0.0001). CONCLUSIONS For infertile patients with High DFI = 30%, using testicular sperm for ICSI significantly increase clinical pregnancy rate as well as Live birth rate. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e172 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Ahmad Hassan AlMalki More articles by this author Haitham T ElBardisi More articles by this author Sami S AlSaid More articles by this author Hassan Burjaq More articles by this author Moza AlBadr More articles by this author Mohamad M Arafa More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...