PD05-09 EFFECTIVENESS OF INDIVIDUAL AND SMALL NUMBER OF HUMAN SPERMATOZOA CRYOPRESERVATION FOR FERTILITY PRESERVATION IN PATIENTS WITH SEVERE MALE FACTOR OF INFERTILITY

Abstract

You have accessJournal of UrologyInfertility: Therapy I1 Apr 2016PD05-09 EFFECTIVENESS OF INDIVIDUAL AND SMALL NUMBER OF HUMAN SPERMATOZOA CRYOPRESERVATION FOR FERTILITY PRESERVATION IN PATIENTS WITH SEVERE MALE FACTOR OF INFERTILITY Jianlin Hu, Jian Sun, Yuan Xu, Zijue Zhu, Xiaofeng Wan, Yuehua Gong, Yan Hong, Huixing Chen, Peng Li, Xiaohong Gao, Zhenbo Zhang, Zheng Li, and Yu Wu Jianlin HuJianlin Hu More articles by this author , Jian SunJian Sun More articles by this author , Yuan XuYuan Xu More articles by this author , Zijue ZhuZijue Zhu More articles by this author , Xiaofeng WanXiaofeng Wan More articles by this author , Yuehua GongYuehua Gong More articles by this author , Yan HongYan Hong More articles by this author , Huixing ChenHuixing Chen More articles by this author , Peng LiPeng Li More articles by this author , Xiaohong GaoXiaohong Gao More articles by this author , Zhenbo ZhangZhenbo Zhang More articles by this author , Zheng LiZheng Li More articles by this author , and Yu WuYu Wu More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2616AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To evaluate the effectiveness of individual and small number of human spermatozoa cryopreservation in patients with severe male factor of infertility and to assess their clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles with frozen-thawed sperm. METHODS We retrospectively analyzed 174 samples of 128 patients who underwent male fertility preservation by individual or small number of human spermatozoa cryopreservation. They came to our center from March to October in 2015, of whom 16 cases were subjected to 16 ICSI cycles with frozen-thawed sperm. We further investigated the rates of sperm recovery, fertilization, cleavage, transferable embryos, good-quality embryos and clinical pregnancy. RESULTS Among the 128 patients, 103 frozen samples were obtained by masturbation in 58 severe oligozoospermia or severe asthenozoospermia cases. A total of 8 samples were obtained by electroejaculation and 3 by conventional testicular sperm extraction (cTESE) in 11 ejaculation disorder (anejaculation or retrograde ejaculation) cases. Moreover, 48 samples were obtained by cTESE in 43 obstructive azoospermia(OA) cases and 5 non-obstructive azoospermia(NOA) cases, and 11 samples by Micro-dissection TESE(MD-TESE) in 11 NOA cases. We use cryotube, 0.25ml straw or cryotip as vectors for small number spermatozoa cryopreservation according to the semen quality and cryopiece for individual spermatozoa cryopreservation. The sperm recover rate was 94.74% (108/114) in 0.25ml straw group and 93.10%(27/29) in cryotip group. A total of 25 samples were frozen by cryopiece and 6 by cryotube without undergoing frozen-thawed tests. As to the outcomes of 16 ICSI cycles, all the frozen sperm samples were recovered successfully. The rates of fertilization, 2PN fertilization, cleavage, transferable and good-quality embryos were 79.64%(133/167), 75.45%(126/167), 97.74%(130/133), 56.92%(74/130), 42.31%(55/130), respectively. Fresh embryos were transferred in 13 of the 16 cycles, with the clinical pregnancy rate of 61.54%(8/13), including 3 singletons and 5 twins. CONCLUSIONS Techniques of individual and small number of human spermatozoa cryopreservation are effective methods of fertility preservation for severe male infertility patients. The clinical outcomes of ICSI cycles are good by using frozen-thawed sperm through these methods. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e172 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Jianlin Hu More articles by this author Jian Sun More articles by this author Yuan Xu More articles by this author Zijue Zhu More articles by this author Xiaofeng Wan More articles by this author Yuehua Gong More articles by this author Yan Hong More articles by this author Huixing Chen More articles by this author Peng Li More articles by this author Xiaohong Gao More articles by this author Zhenbo Zhang More articles by this author Zheng Li More articles by this author Yu Wu More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...