PD04-09 BROWN ADIPOCYTES SUPPRESS KIDNEY STONE FORMATION VIA HEAT-PRODUCING PROTEIN, UNCOUPLING PROTEIN 1

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology I (PD04)1 Apr 2020PD04-09 BROWN ADIPOCYTES SUPPRESS KIDNEY STONE FORMATION VIA HEAT-PRODUCING PROTEIN, UNCOUPLING PROTEIN 1 Teruaki Sugino*, Yutaro Tanaka, Rei Unno, Kazumi Taguchi, Shuzo Hamamoto, Ryosuke Ando, Atsushi Okada, Tohru Mogami, Hitoshi Yamashita, and Takahiro Yasui Teruaki Sugino*Teruaki Sugino* More articles by this author , Yutaro TanakaYutaro Tanaka More articles by this author , Rei UnnoRei Unno More articles by this author , Kazumi TaguchiKazumi Taguchi More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Ryosuke AndoRyosuke Ando More articles by this author , Atsushi OkadaAtsushi Okada More articles by this author , Tohru MogamiTohru Mogami More articles by this author , Hitoshi YamashitaHitoshi Yamashita More articles by this author , and Takahiro YasuiTakahiro Yasui More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000824.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Our recent studies have revealed that adipocytokine secreted by adipocytes are important for the formation of kidney stones. Brown adipocytes are reported to suppress inflammatory adipocytokine expression via a specific protein—uncoupling protein 1 (UCP1). In this study, we investigated the effect of brown adipocytes and UCP1 on kidney stone formation. METHODS: Study 1: Nine-week-old male C57BL/6 mice underwent sham or brown adipose tissue transplant surgery (sham group, transplant group, n = 6 in each group). Three weeks after surgery, renal crystal deposits were introduced via daily intra-abdominal injections of 80 mg/kg glyoxylate (GOX). Study 2: Renal crystal deposits were introduced via daily intra-abdominal injections of 80 mg/kg GOX in wild-type and defective type 8-week-old male UCP1-deficient mice (B6.129-Ucp1tm1Kz/J) (WT group, KO group, n = 6 in each group). Fat tissues, kidneys, and 24-hour urine samples of the mice were collected in both studies. RESULTS: Study 1: In the transplant group, the amount of renal crystal deposits was significantly lower (3.28 times) than that in the sham group (Fig. 1a), and lower expression of inflammatory markers, such as chemokine (C-C motif) ligand 2 (Ccl2), EGF module-containing mucin-like receptor 1 (Emr1), tumor necrosis factor (Tnf), and secreted phosphoprotein 1 (Spp1)was observed in the kidney. Inductive brown adipocytes were observed in the perirenal fat tissue in the transplant group. Study 2: In the KO group, the amount of renal crystal deposits was significantly higher (3.28 times) than that in the WT group (Fig. 1b), and higher expression of Emr1, Tnf, and Spp1 was observed in the kidney. Infiltration of inflammatory cells was strongly observed in brown adipose tissue in the KO group. There were no significant differences in the 24-hour urine samples throughout the two studies. CONCLUSIONS: Brown adipose tissue transplantation suppressed renal crystal formation via anti-inflammatory effects, and UCP1 would be necessary in this mechanism. Brown adipocytes can be the therapeutic target for kidney stone formation. Source of Funding: none © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e81-e82 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Teruaki Sugino* More articles by this author Yutaro Tanaka More articles by this author Rei Unno More articles by this author Kazumi Taguchi More articles by this author Shuzo Hamamoto More articles by this author Ryosuke Ando More articles by this author Atsushi Okada More articles by this author Tohru Mogami More articles by this author Hitoshi Yamashita More articles by this author Takahiro Yasui More articles by this author Expand All Advertisement PDF downloadLoading ...